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The general objectives of our research, presented in this series of papers, were to develop a computational model of the brain stem respiratory neural network and to explore possible neural mechanisms that provide the genesis of respiratory oscillations and the specific firing patterns of respiratory neurons. The present paper describes models of single(More)
We have developed a bioinformatics tool named PAINT that automates the promoter analysis of a given set of genes for the presence of transcription factor binding sites. Based on coincidence of regulatory sites, this tool produces an interaction matrix that represents a candidate transcriptional regulatory network. This tool currently consists of (1) a(More)
Although the amygdala complex has long been known to exert a profound influence on cardiovascular activity, the neuronal and connectional substrate mediating these influences remains unclear. This paper describes a direct amygdaloid projection to medullary sensory and motor structures involved in cardiovascular regulation, the nucleus of the solitary tract(More)
Uptake, replication, and transneuronal passage of a swine neurotropic herpesvirus (pseudorabies virus, PRV) was evaluated in the rat CNS. PRV was localized in neural circuits innervating the tongue, stomach, esophagus and eye with light microscopic immunohistochemistry. In each instance, the distribution of PRV-immunoreactive neurons was entirely consistent(More)
Previous light microscopic work in the rat has demonstrated that many dendrites of vagal gastric motoneurons extend beyond the cytoarchitectural boundaries of the dorsal motor nucleus of the vagus (DMV) into the nucleus of the solitary tract (NST), where they overlap with the central terminal field of vagal gastric primary sensory neurons (Shapiro and(More)
1. We explore the roles of conductances in Hodgkin-Huxley (HH) models using a method that allows the explicit linking of HH model input-output behavior to parameter values for maximal conductances, voltage shifts, and time constants. The procedure can be used to identify not only the parameter values most critical to supporting a neuronal activity pattern(More)
To study the selectivity of neurotrophic actions in the brain, we analyzed the actions of several known growth factors on septal cholinergic, pontine cholinergic, and mesencephalic dopaminergic neurons in culture. Similar to nerve growth factor (NGF), basic fibroblast growth factor (bFGF) stimulated choline acetyltransferase activity in septal cultures. In(More)
We defined the central circuit innervating various regions of the rat heart using a neurotropic herpesvirus as a transneuronal tracer. Location of viral antigens in the brain after cardiac injection of three strains of pseudorabies virus (PRV) provided insight into vagal preganglionic neurons and their connected interneurons. At short survival times,(More)
Gene expression profiles are an increasingly common data source that can yield insights into the functions of cells at a system-wide level. The present work considers the limitations in information content of gene expression data for reverse engineering regulatory networks. An in silico genetic regulatory network was constructed for this purpose. Using the(More)
We injected the anterograde fluorescent tracer 1,1'-dioleyl-3,3,3',3'-tetramethylindocarbocyanine methanesulfonate (DiI) into the dorsal motor nucleus of the vagus (DmnX), counterstained the cardiac ganglia with Fluorogold (FG), and used confocal microscopy to examine the distributions and different types of DmnX fibers in wholemounts of the atria. We also(More)