James S. Lovett

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Freshly harvested zoospores of Blastocladiella emersonii begin to germinate about 15 min after inoculation into a defined growth medium at a density of 10(6) zoospores per ml. Flagellum retraction accompanies encystment, and dispersal of the ribosomal nuclear cap takes place shortly thereafter. The primary rhizoid begins to emerge at 25 to 30 min and starts(More)
Lovett, James S. (Purdue University, West Lafayette, Ind.). Chemical and physical characterization of "nuclear caps" isolated from Blastocladiella zoospores. J. Bacteriol. 85:1235-1246. 1963.-Electron micrographs of Blastocladiella zoospores have shown the nuclear cap to contain essentially all of the small (250 to 300 A) electron-dense particles of the(More)
The factors responsible for the regulation of protein synthesis in the zoospores of Blastocladiella emersonii were studied by means of cell fractionation and in vitro assays. Charged transfer ribonucleic acid (tRNA) and aminoacyl-tRNA synthetases were found both inside the membrane-bound, ribosomal nuclear cap, and in the extracap cytoplasm. Ribosomes(More)
The tubulin proteins of Blastocladiella emersonii have been characterized, and the pool sizes of soluble tubulins measured to evaluate turnover during early development. The axonemal tubulins and soluble tubulin dimers were typical of tubulin proteins from other eukaryotes. [3H]cholchicine binding assays were used to estimate the soluble tubulin pools of(More)
Samples from synchronized cultures of Rlastorlacliella e m e ~ s o n i i were examined by electron microscopy from t,he late log phase to the completioli of zoospore differentiation, Log-phase plants contain t,he usual cytoplasmic organelles but also have an unusual system of large tubules ca. 45 mp diam that ramify in organized bundles throughout the(More)