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— This paper describes a data set collected by an autonomous ground vehicle testbed, based upon a modified Ford F-250 pickup truck. The vehicle is outfitted with a professional (Applanix POS-LV) and consumer (Xsens MTi-G) inertial measurement unit (IMU), a Velodyne 3D-lidar scanner, two push-broom forward looking Riegl lidars, and a Point Grey Ladybug3(More)
This paper reports on a mutual information (MI) based algorithm for automatic extrinsic calibration of a 3D laser scanner and optical camera system. By using MI as the registration criterion, our method is able to work in situ without the need for any specific calibration targets, which makes it practical for in-field calibration. The calibration parameters(More)
We have developed and optimized a 96-well microtiter plate assay, based on the reduction of alamarBlue, to assess the efficacies of much needed new antimicrobials against Acanthamoeba species. This assay has been optimized for determination of drug efficacy against two potentially pathogenic species, Acanthamoeba castellanii and Acanthamoeba polyphaga, and(More)
We propose an approach for external calibration of a 3D laser scanner with an omnidirectional camera system. The utility of an accurate calibration is that it allows for precise co-registration between the camera imagery and the 3D point cloud. This association can be used to enhance various state of the art algorithms in computer vision and robotics. The(More)
To explore the potential for use of ligand-conjugated nanocrystals to target cell surface receptors, ion channels, and transporters, we explored the ability of serotonin-labeled CdSe nanocrystals (SNACs) to interact with antidepressant-sensitive, human and Drosophila serotonin transporters (hSERT, dSERT) expressed in HeLa and HEK-293 cells. Unlike(More)
Semiconductor quantum dots are quickly becoming a critical diagnostic tool for discerning cellular function at the molecular level. Their high brightness, long-lasting, size-tunable, and narrow luminescence set them apart from conventional fluorescence dyes. Quantum dots are being developed for a variety of biologically oriented applications, including(More)
OBJECTIVES Previous studies have reported the ability of several phospholipid analogues to successfully inhibit the growth of Acanthamoeba species in vitro. This study tests further phospholipid analogues, either as free drug or in liposomal formulations, and unlike previous studies, examines their comparative toxicities to mammalian cells. METHODS The(More)
Magic-sized cadmium selenide (CdSe) nanocrystals have been pyrolytically synthesized. These ultra-small nanocrystals exhibit broadband emission (420-710 nm) that covers most of the visible spectrum while not suffering from self absorption. This behavior is a direct result of the extremely narrow size distribution and unusually large Stokes shift (40-50 nm).(More)
A simple treatment method using formic acid has been found to increase the fluorescence quantum yield of ultrasmall white light-emitting CdSe nanocrystals from 8% to 45%. Brighter white-light emission occurs with other carboxylic acids as well, and the magnitude of the quantum yield enhancement is shown to be dependent on the alkyl chain length.(More)