James R. Henderson

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LIM domains are double zinc-finger motifs found in many proteins that play central roles in cell differentiation. Members of the cysteine-rich protein (CRP) family display two LIM domains and are implicated in muscle development. Here we describe the characterization of one member of this family, CRP1, in the mouse. We have isolated and sequenced murine(More)
In passing from islets to the exocrine part of the gland, pancreatic capillaries change their character. Islet capillaries are significantly wider than exocrine (5.27 micrometers, diameter compared with 4.35 micrometers), thinner walled and have many more fenestrations micrometers-1 of endothelium (1.3 compared with 0.13). The point of transition at the(More)
Members of the cysteine-rich protein (CRP) family are evolutionarily conserved Lin-11, Isl-1, Mac-3 (LIM) domain proteins that have been implicated in cell differentiation. Here we describe the expression pattern of the CRP family member CRP2 in mouse. Unlike other CRP family members, which are expressed primarily in muscle, CRP2 is more broadly expressed.(More)
A new integrated optical and electrochemical sensor system for simultaneous monitoring of intra- and extracellular superoxide (O(2)(-)) was developed using an array-based cell chip. For in vitro assays, A172 human glioblastoma cells were transferred into the cell chip and stimulated by phorbol 12-myristate 13-acetate (PMA). Intracellular O(2)(-) generation(More)
1. The concentration of insulin in the blood of fifteen fasting baboons, anaesthetized with pentobarbitone sodium, was measured by a specific radio-immunoassay method.2. The mean resting concentration of insulin was: inferior vena cava (IVC) 22 muu./ml. and splenic vein (after splenectomy) 140 muu./ml.3. Subdiaphragmatic stimulation of the cut right vagal(More)
A new approach for an amperometric array sensor platform employing arrays of sensors in a 24-well cell culture plate format has been developed for simultaneous in vitro determination of nitric oxide (NO) and superoxide free radicals (O(2)(-)) produced by stimulated cells. The work reported focuses on the direct, real-time monitoring of extracellular(More)
A combined, optospectroscopic and electrochemical assay system for the simultaneous monitoring of intra- and extracellular production of biologically important species has been developed and assessed. The present model system evaluates intra- and extracellular nitric oxide produced by stimulated glioblastoma multiform cell line (A172). The production of(More)