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Evidence for plasmid- and chromosome-borne multiple nif genes in Rhizobium fredii.
Rhizobium fredii is a fast-growing rhizobium isolated from the primitive Chinese soybean cultivar Peking and from the wild soybean Glycine soja. This rhizobium harbors nif genes on 150- toExpand
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Symbiotic Effectiveness and Host-Strain Interactions of Rhizobium fredii USDA 191 on Different Soybean Cultivars.
Nodulation, acetylene reduction activity, dry matter accumulation, and total nitrogen accumulation by nodulated plants growing in a nitrogen-free culture system were used to compare the symbioticExpand
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Detection of genetic variation in Bradyrhizobium japonicum USDA 110 variants using DNA fingerprints generated with GC rich arbitrary PCR primers
Bradyrhizobium japonicum USDA 110 has been shown to contain several genetically similar naturally occurring colony morphology variants. These variants differ in symbiotic nitrogen fixation abilityExpand
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Isolation and characterization ofMicrococcus plasmids
Plasmids were detected in a small to moderate percentage of strains in the speciesMicrococcus kristinae (7%)M. agilis (20%),M. luteus (20%),M. varians (23%),M. nishinomiyaensis (41%), andM. roseusExpand
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Detection and Transcription of Toxin DNA in a Nontoxigenic Strain of Clostridium difficile
Abstract. Genomic DNA from three Clostridium difficile strains was analyzed by PCR for DNA sequences encoding toxin A (tcdA) and toxin B (tcdB). Toxigenic control strain VPI 10463 possessed tcdA,Expand
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In vivo labeling of Escherichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin.
The primary amine coupling reagents succinimidyl-6-biotinamido-hexanoate (NHS-A-biotin) and sulfosuccinimidyl-6-biotinamido-hexanoate (NHS-LC-biotin) were tested for their ability to selectivelyExpand
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Effect of Sym Plasmid Curing on Symbiotic Effectiveness in Rhizobium fredii.
A mutant, USDA 206C, of Rhizobium fredii USDA 206 was obtained by passage on acridine plates. This mutant was cured of its 197-megadalton Sym plasmid but retained its symbiotic effectiveness.Expand
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Genetic Variation in Two Cultures of Bradyrhizobium japonicum 110 Differing in Their Ability to Impart Drought Tolerance to Soybean
Abstract. The polymerase chain reaction with arbitrary primers (RAPD) discriminated between two separately maintained cultures of Bradyrhizobium japonicum USDA 110 differing in symbiotic performanceExpand
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Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedlings : a species comparison.
The biosynthesis of the chlorophyll a/b binding protein associated with photosystem II (LHC-II) was characterized during light-induced greening of etiolated barley (Hordeum vulgare [L.] cv Boone),Expand
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Restriction Endonuclease and nif Homology Patterns of Bradyrhizobium japonicum USDA 110 Derivatives With and Without Nitrogen Fixation Competence.
DNAs from Bradyrhizobium japonicum USDA 110 derivatives that differ in nitrogen-fixing ability produced similar electrophoretic patterns with five different restriction enzymes. Our data support theExpand
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