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Molecular noise in gene expression can generate substantial variability in protein concentration. However, its effect on the precision of a natural eukaryotic circuit such as the control of cell cycle remains unclear. We use single-cell imaging of fluorescently labelled budding yeast to measure times from division to budding (G1) and from budding to the(More)
BACKGROUND The capacity of cancer cells to undergo epithelial mesenchymal trans-differentiation has been implicated as a factor driving metastasis, through the acquisition of enhanced migratory/invasive cell programs and the engagement of anti-apoptotic mechanisms promoting drug and radiation resistance. Our aim was to define molecular signaling changes(More)
Cell cycle "Start" in budding yeast involves induction of a large battery of G1/S-regulated genes, coordinated with bud morphogenesis. It is unknown how intra-Start coherence of these events and inter-Start timing regularity are achieved. We developed quantitative time-lapse fluorescence microscopy on a multicell-cycle timescale, for following expression of(More)
The breast cancer susceptibility gene BRCA1 encodes a protein implicated in the cellular response to DNA damage, with postulated roles in homologous recombination as well as transcriptional regulation. To identify downstream target genes, we established cell lines with tightly regulated inducible expression of BRCA1. High-density oligonucleotide arrays were(More)
Epithelial-mesenchymal transition (EMT) is an important contributor to the invasion and metastasis of epithelial-derived cancers. While considerable effort has focused in the regulators involved in the transition process, we have focused on consequences of EMT to prosurvival signaling. Changes in distinct metastable and 'epigentically-fixed' EMT states were(More)
Hepatitis C virus (HCV) is a public health crisis, affecting over 170 million people by 2011. Conventional treatment, interferon alpha with ribavirin, can often be ineffective and may lead to severe side effects, and this lack of effective treatment poses significant challenges to patients, healthcare providers, and public health officials. As ii a result,(More)
  • Darby E Grande, James C Bean, Sebastian Fixson, Gregory A Keoleian, Assistant Professor, Dushyant Sharma +5 others
  • 2004
ACKNOWLEDGEMENTS I am truly humbled by the many contributions that led to the realization of this work. The first thanks are due to my advisor, James Bean, whose breadth of knowledge , insightful mentoring, and example of leadership have kept me working toward this goal. I also thank my committee members Sebastian Fixson, Greg Keoleian and Dushyant Sharma(More)
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