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Corticotropin releasing factor is a 41 amino acid peptide that is present in afferent systems that project to the cerebellum. In the adult, this peptide modulates the activity of Purkinje cells by enhancing their responsiveness to excitatory amino acids. Two different types of corticotropin releasing factor receptors, designated type 1 and type 2, have been(More)
Immunohistochemistry and high-resolution autoradiography were used to analyze the distribution and synaptic organization of serotonin (5HT) - containing elements in the inferior olivary complex of the opossum. Immunoreactive beaded varicosities are present throughout the olivary complex. The densest 5HT immunostaining is present in subnucleus b of the(More)
The motor nucleus of the opossum trigeminal nerve consists of a main body and a small dorsomedial cell cluster. The cell bodies form a unimodal population with areas that range from 150-2700 mum2. Golgi impregnations reveal that each neuron has three to six primary dendrites which radiate in all planes from the cell body. Within 300 mum from the soma, the(More)
Silver enhancement of small gold particles can be used with pre-embedding immunocytochemistry to analyze the distribution of label over cell organelles. We have developed a method that improves tissue morphology, has good penetration of reagents, and allows greater control of silver enhancement of 1.4-nm gold. In this study we analyzed the distribution of(More)
Corticotropin releasing factor (CRF) is a 41 amino acid peptide that has been localized throughout the mouse cerebellum on postnatal day (P0). The wide-spread distribution of CRF within this brain region at birth suggests that it likely is present during embryonic stages of development. Thus, the intent of this study was to use immunohistochemical(More)
This study reports 1) a nonhomogeneous distribution of three morphologically distinct, corticotropin-releasing factor (CRF)-immunoreactive axonal phenotypes within the cerebellum of the opossum: climbing fibers, mossy fibers, and beaded fibers within the ganglionic plexus; 2) the existence of CRF binding sites within the cerebellar cortex; and 3) the(More)
Corticotropin-releasing factor (CRF) is present in climbing and mossy fibers and both have a distinct pattern of distribution in the adult cerebellar cortex. The intent of this developmental study is to determine when the lobular pattern of CRF distribution emerges, and to analyze the morphogenesis of CRF immunoreactive climbing and mossy fibers in(More)
Interleukin-6 (IL-6) type cytokines show functional redundancy in the immune, hematopoietic, and nervous system, which is believed to result from sharing of the signal transducing receptor gp130. IL-6 type cytokines and their binding receptors have been localized in the adult cerebellum. However, the cellular localization and developmental regulation of(More)
Intracellular recordings have been obtained from neurons in lobule V of the cat's vermis, which were identified as basket cells following intracellular injections of HRP. Stimulation of the inferior cerebellar peduncle or peripheral nerves elicited an initial depolarizing and subsequent hyperpolarizing response. Neither potential could be graded with(More)