James J. Stragand

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We categorized established human colon carcinoma cell lines into three biological groups. Our studies were performed on six colorectal cancer lines representing the proposed three groups. Group I consisted of two lines designated LoVo and SW 48; Group II comprised two lines called SW 480 and SW 620; and Group III was represented by lines SW 403 and SW 1116.(More)
Following a period of fasting, feeding a normal diet results in a burst of DNA synthesis in the crypts of the colonic epithelium. This is due largely to a prompt entry of cells, blocked in G1, into S. Peak levels of S cellularity exceed 4 times the fasting, and 2 times the normal fed control values. Refeeding a low residue diet (soluble casien, glucose and(More)
The cellular kinetics of human mammary tumors were studied by in vitro methods. These techniques include single 3HTdR labeling to measure the 3HTdR LI, double labeling with 3HTdR and 14CTdR to measure DNA synthesis time, and an estimation of the growth fraction by the PDP index. Calculations of the potential doubling time and cell cycle time were made from(More)
To assess the effects of guar gum on colonic microbial fermentation and cancer development, azoxymethane-treated rats were fed a partially hydrolyzed guar or control diet. Anaerobic fecal incubations were conducted at 8-wk intervals, either without added substrate or with cornstarch or hydrolyzed guar as substrates. Short-chain fatty acids in colonic(More)
The present studies were initiated to investigate the effectsof methylprednisolone (MP)on the growthand cell kinetics of C3H/HeJ spontaneousmammary tumor. MP given every 12 hr in 9 doses at 10 or 20 mg/kg/dose resulted In temporary stasis of tumor growth with liftle or novolumeregression.Cell kineticparameterswere deter minedbyIn vitromethodsafter MP every(More)
The biological and cell kinetic properties of the poorly differentiated human colonic adenocarcinoma cell line SW 620 grown as xenografts in BALB/c athymic mice are described. The SW 620 cells consistently produced tumors when inoculated i.v., i.p., and s.c. The lowest cell inoculum requirements were seen i.p. where 10(7) cells produced a 100% incidence.(More)
Recent studies have shown that the rate of colonic cell renewal can be altered through fasting and refeeding, which produces a marked depression and transient stimulation, respectively. In the present study, the role of physical versus nutritional stimulation in the colonic fasting-refeeding response and the renewal of the functional colonic compartment(More)