James H. Chin

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Amyloid beta (Abeta) protein, a 39-43 amino acid peptide deposited in brains of individuals with Alzheimer's disease (AD), has been shown to interact directly with a number of receptor targets including neuronal nicotinic acetylcholine receptors (nAChRs) and glutamate receptors. In this study, we investigated the synaptic effects of Abeta(1-42) on(More)
Nociceptin/orphanin FQ (N/OFQ) is an endogenous opioid-like heptadecapeptide that plays an important role in a variety of physiological functions. N/OFQ and its receptor opioid receptor-like orphan receptor-1 are abundant in the diagonal band of Broca (DBB), a basal forebrain nucleus where the loss of cholinergic neurons is linked to memory and spatial(More)
Beta-amyloid, a 39-43 amino acid peptide, may exert its biological effects via neuronal nicotinic acetylcholine receptors. Using the ratiometric dye, fura-2, we examined the effect of soluble beta-amyloid(1-42) on the concentration of intracellular Ca(2+) ([Ca(2+)](i)) in acutely dissociated rat basal forebrain neurons. Focal applications of nicotine(More)
Blunted cAMP responses to beta-adrenergic agonists play a major role in diminished smooth muscle relaxation in blood vessels from older animals, although the mechanisms remain uncertain. A diminished cAMP response could potentially arise from changes in the expression of adenylyl cyclase-coupled G proteins, such as a diminished expression of Gs or an(More)
beta-Adrenergic agonists stimulate cyclic 3',5'-adenosine monophosphate (cAMP) accumulation and relaxation in vascular smooth muscle; both of these responses decline in rat aorta with increasing age. To ascertain whether the deficit in beta-receptor stimulated cAMP accumulation persists in isolated aortic smooth muscle cells, the effect of isoproterenol on(More)
Both vascular relaxation and cAMP accumulation mediated by beta adrenergic agonists declines with increasing age. We have developed a method to measure the biologically relevant cAMP bound to the regulatory subunits of cAMP-dependent protein kinase in rat aorta. In homogenates of rat aorta, binding of [3H]cAMP was saturable with a Kd of 8.0 +/- 1.5 nM; the(More)
Calcium ions are important regulators of a wide range of cellular functions [1]. These include stimulus response events such as muscle contraction and the release of hormones and neurotransmitters, as well as the regulation of enzyme activities, transport phenomena, and membrane permeabilities. Moreover. calcium has been implicated in the modulation of(More)
2-Chloro[3H]adenosine, a stable analog of adenosine, was used to investigate the presence of adenosine receptors in rat hippocampal membranes that may mediate the depressant effects of adenosine on synaptic transmission in this tissue. Equilibrium binding studies reveal the presence of a previously undescribed class of receptors with a KD of 4.7 microM and(More)