James Fullton

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The rate of Cl- secretion by human airway epithelium is determined, in part, by apical cell membrane Cl- conductance. In cystic fibrosis airway epithelia, defective regulation of Cl- conductance decreases the capability to secrete Cl-. Here we report that extracytosolic ATP in the luminal bath of cultured human airway epithelia increased transepithelial Cl-(More)
Transepithelial Na+ absorption is increased two to three times in cystic fibrosis (CF) compared with normal (NL) airway epithelia. This increase has been associated with a higher Na+ permeability of the apical membrane of airway epithelial cells. Because Na+ absorption is electrogenic and abolished by amiloride, Na+ channels are thought to dominate the(More)
Respiratory mechanical parameters were computed from forced random noise impedance data in normal adults (group 1), asymptomatic smokers (group 2), and patients with obstructive pulmonary disease (group 3). Mean values for all derived parameters were significantly different (p less than 0.025) for group 3. Mean values of resonant frequency, the ratio of(More)
We used patch-clamp techniques to study the channels that underlie the Na+ conductance of the apical membrane of human normal nasal epithelial cells. Cells were cultured on permeable supports and studied after confluence. In 172 of 334 (52%) excised membrane patches, we observed 20-pS Na(+)-permeable channels that do not discriminate between Na+ and K+(More)
Retrograde catheter and forced random noise techniques were combined to study the distribution of resistance and compliance in dogs following the inhalation of aerosols containing 2.5 and 5.0 mg/ml of histamine. Mean base-line peripheral resistance was 0.367 cmH2O . l-1 . s, agreeing with previous estimates. After correction for the endotracheal tube, the(More)
The endogenous Cl- conductance of Spodoptera frugiperda (Sf9) cells was studied 20-35 h after plating out of either uninfected cells or cells infected by a baculovirus vector carrying the cloned beta-galactosidase gene (beta-Gal cells). With the cation Tris+ in the pipette and Na+ in the bath, the reversal potential of whole-cell currents was governed by(More)
The frequency dependence of total respiratory impedance during spontaneous breathing was measured repeatedly in 16 children (3--5 yr old) over a 3-mo period using forced random noise and spectral analysis. Total respiratory resistance, compliance, and inertance, which were calculated fromthe impedance data using regression analysis with a second-order(More)
The correlation between airway resistance (Raw) measured in a plethysmograph and three respiratory resistance parameters measured by forced random noise was evaluated. Forced random noise resistance parameters were the average resistance between 5 and 9 hz (R5-9), the average resistance between 20 and 24 Hz (R20-24), and the extrapolated resistance at 1 Hz(More)
Total respiratory resistance (Rrs) and the fraction of this resistance located peripherally, (Fp) were measured in a group of children (3--5 yr old). The technique used forced random noise to characterize the frequency dependence of effective resistance and an algorithm to compute Rrs and Fp from these data. The overall mean value +/- SD for Fp was 0.534(More)