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Using permeabilized chromaffin cells and the fluorescent probe Quin 2 (an indicator of free Ca2+), we found that inositol trisphosphate (IP3) specifically triggered an immediate and dose-dependent release of Ca2+ from intracellular stores. Desensitization of the response was observed at nonsaturating concentrations of inositol trisphosphate and(More)
Neutrophils labelled with chlorotetracycline (commonly employed as a probe for membrane-bound calcium), underwent rapid decreases in fluorescence upon exposure to N-formylmethionylleucylphenylalanine (more than 1 nM). This decrease was maximal at 1 min and was followed by partial recovery by 3 min. When neutrophils were stimulated with(More)
Extracts of human neutrophils were examined for phosphodiesterase activity using a radiochemical assay. As reported by other investigators, both high- and low-Km forms of the enzyme were found. Although calmodulin could be measured in these extracts, human neutrophil phosphodiesterase proved not to be calmodulin dependent. Activity of the neutrophil(More)
The adhesion molecules known as selectins mediate the capture of neutrophils from the bloodstream. We have previously reported that ligation and cross-linking of L-selectin on the neutrophil surface enhances the adhesive function of beta(2)-integrins in a synergistic manner with chemotactic agonists. In this work, we examined degranulation and adhesion of(More)
In this review of secretion by neutrophils, we have given a detailed description of the characteristics of the exocytotic responses of these cells to a variety of stimuli. Secretion of the contents of the two types of granules follows different rules. Azurophil granules are discharged only when neutrophils interact with phagocytizable particles, whereas(More)