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The toxic acetate analogue monofluoroacetic acid was employed to isolate Arabidopsis tDNA-tagged plants deficient in their ability to utilize or sense acetate. Several tDNA-tagged lines were isolated, including two that were determined to be allelic to an EMS-mutagenized line denoted acn1 for ac non-utilizing. Following conventions, the tDNA-tagged mutants(More)
We report the successful grafting of embryonic (newborn) rat retina into a lesion site (die-back zone) of an adult retina with a corresponding 90-100% survival rate. A penetrating lesion was made through the sclera, choroid and retina on the superior surface of the host eye and closed with microsutures. The lesion site was either allowed to stabilize for 5(More)
Retinal pigment epithelial (RPE) cells isolated from 6-8-day-old pigmented Long-Evans rat eyes were successfully grafted onto Bruch's membrane in albino Sprague-Dawley hosts ranging in age from 10 days postnatal to young adulthood. Injections of RPE cells were made into the subretinal space using a lesion paradigm which penetrates through the dorsal surface(More)
Photoreceptor cells in Royal College of Surgeons (RCS) rats with inherited retinal dystrophy can be rescued by the transplantation of normal, wild-type retinal pigment epithelial (RPE) cells, if done before photoreceptor cell death. In the present study, we have examined several metabolic features of rescued photoreceptors and transplanted RPE cells at(More)
We have studied the time course of the expression of glial fibrillary acidic protein (GFAP) and S-100 developmentally regulated proteins in host and graft tissue after transplantation of rat E15 retina to retinal lesion sites of adult rat hosts. Host Müller cell reactivity (GFAP staining) appeared in the peripheral retina 4.5 h after the lesion apparently(More)
The rat retina can be successfully grafted within a long time period which extends into the first 2 weeks of postnatal life. Postnatal grafts taken 1-2 days (PN 1-2) after birth demonstrate no significant differences in their ability to form successful grafts. However, grafting success begins to diminish gradually starting between PN 2-4 and reaches a low(More)
Exercise induces mobilisation of CD8(+) T lymphocytes (CD8TL) into the peripheral blood. This response is largely confined to effector-memory CD8TLs: antigen experienced cells which have a strong tissue-homing and effector potential. This study investigated whether effector-memory cells also account for the CD8TL egress from peripheral blood following(More)
Embryonic day-15 mouse retinas were grafted into an adult rat retinal lesion site and allowed to survive for 9 and 30 days. Grafted animals received either no Cyclosporin A treatment, treatment for the first 10 days of transplantation or continuous treatment. In a second experimental set up, postnatal day-21 rat retinas were grafted under the same(More)
The present study examined whether a high protein diet prevents the impaired leukocyte redistribution in response to acute exercise caused by a large volume of high-intensity exercise training. Eight cyclists (VO2max: 64.2±6.5mLkg(-1)min(-1)) undertook two separate weeks of high-intensity training while consuming either a high protein diet(More)