James C. H. Tan

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PURPOSE To directly visualize the live cellularity of the intact human trabecular meshwork (TM) and quantitatively analyze tissue viability in situ. METHODS Human donor corneoscleral rims were sectioned immediately before intravital dye incubation to label nuclei (Hoechst 33342 & propidium iodide [PI]); cytosol (CellTracker Red CMTPX, calcein AM); and(More)
PURPOSE To develop an in situ model to study biological responses and glaucoma pathology in the human trabecular meshwork (TM). Characteristic TM cell- and glaucoma-associated markers were localized in situ in relation to the tissue's autofluorescent structural extracellular matrix (ECM) by two-photon excitation fluorescence optical sectioning (TPEF). (More)
PURPOSE In situ 2-photon excitation fluorescence (TPEF) of the human trabecular meshwork (TM) reveals beams of heterogeneous autofluorescence (AF) comprising high intensity fluorescent fibers (AF-high) on a background of lower intensity fluorescence (AF-low). To determine the sources of this AF heterogeneity, we imaged human TM to characterize AF, second(More)
PURPOSE Reference plane description of the neuroretinal rim in scanning laser tomography should correctly represent optic nerve morphology. We evaluated how well rim area analysis by different reference planes agreed with the appearance of rim area in disc images. METHODS Three expert observers subjectively and repeatedly analyzed rim area in Heidelberg(More)
PURPOSE To identify and characterize 'reversal' of optic nerve cupping following intraocular pressure (IOP) lowering in scanning laser tomography (SLT) longitudinal image series. METHODS Modification was made to a previously described analytical approach to longitudinally study putatively increased rim area following IOP lowering. Sustained IOP reduction(More)
PURPOSE To characterize the three-dimensional (3-D) structure of the human trabecular meshwork (TM) by two-photon excited (TPEF) autofluorescence (AF) and optical sectioning without conventional histologic embedding and sectioning. METHODS Viable human ex vivo explants of the anterior chamber angle containing the aqueous humor drainage tissue in situ were(More)
PURPOSE OF REVIEW The state of the actin cytoskeleton and adhesions of trabecular meshwork cells are important determinants of fluid outflow through the trabecular meshwork. Dysregulation of these subcellular structures or cell loss itself, is expected to adversely affect aqueous humour dynamics and intraocular pressure. This article reviews recent research(More)
The cells of the various organ systems in humans are subject to mechanical forces to which they must respond. Here the authors review what is known of the ways in which the cells of animals, ranging from the prokaryotic to humans, sense and transduce mechanical forces to respond to such stimuli. In what way this pertains to the eye, especially with respect(More)
PURPOSE To determine reasons for rim area variability in scanning laser tomography. METHODS Regional rim area variability from testing in same and different visits and by same and different observers was characterized in 30 normal and 42 glaucomatous eyes. Variations in (1) optic nerve head (ONH) surface geometry (center of gravity: X, Y, Z), (2) image(More)