James A Darby

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Several recently-derived monoclonal antibodies (mcabs) that bind with a high degree of specificity to membrane antigens of osteoclasts were tested for their effects on the function of giant cells from osteoclastomas. None of the antibodies stimulated bone resorption when incubated with giant cells on bone slices, nor did we find any evidence that the mcabs(More)
The cell-free endocranial surface of young adult rat parietal bones was used as a substrate for bone cell-derived mammalian collagenase. Incubation of parietal bones in a concentration of enzyme comparable to that secreted by osteoblastic cells in vitro caused destruction of surface osteoid, and resulted in exposure of mineral onto the bone surface. Bones(More)
BACKGROUND Ethyl formate formulations are being considered to replace methyl bromide for fast grain disinfestation. Grain adsorbs ethyl formate rapidly, which can result in inadequate fumigation concentrations and unacceptable grain residues. A model of ethyl formate sorption kinetics will enable fumigation approaches to be determined that meet(More)
We previously found that calcitonin (CT), which inhibits osteoclastic bone resorption, abolished the cytoplasmic motility of isolated osteoclasts. The transition from motility to immotility was accompanied by a characteristic change in cell shape which coincided with the loss of pseudopodial ruffling activity and gradual cytoplasmic retraction. In this(More)
Osteoclasts disaggregated from neonatal rat long bones and incubated on plastic or glass substrates were found to release a considerable proportion of tartrate-resistant acid phosphatase into culture supernatants. Enzyme release was detectable in the supernatant medium of cultures containing as few as ten cells after 1 hr of incubation and proceeded in a(More)
BACKGROUND Most fumigants are adsorbed by grain at differing rates depending on the fumigant or grain type. Sorption can reduce the concentrations of fumigation doses to sublethal levels before grain has been disinfested. A model to predict fumigant losses due to sorption in industrial scenarios is needed. RESULTS This work reviews the kinetics of grain(More)
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