James A. Brouillette

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We are developing a genetic map of the dog based partly upon markers contained within known genes. In order to facilitate the development of these markers, we have used polymerase chain reaction (PCR) primers designed to conserved regions of genes that have been sequenced in at least two species. We have refined the method for designing primers to maximize(More)
Nucleotide diversity (π), the average number of base differences per site for two homologous sequences randomly selected from a population, is an important parameter used to understand the structure and history of populations. It is also important for determining the feasibility of developing a genetic map for a species from single nucleotide polymorphisms(More)
Identification of single nucleotide polymorphisms (SNPs) by DNA sequence comparison across breeds is a strategy for developing genetic markers that are useful for many breeds. However, the heterozygosity of SNPs identified in this way might be severely reduced within breeds by inbreeding or genetic drift in the small effective population size of a breed(More)
Source/description: PCR primers were designed based on the canine rod transducin alpha cDNA sequence (Genbank accession no. Z69597). A single nucleotide polymorphism (G/A) was found within the amplified sequence. A new primer was designed with a single nucleotide mismatch to create a Tth111I restriction site in the presence of the G allele (allele 2), but(More)
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