Jake R. Lindquist

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Spectral diversity of fluorescent proteins, crucial for multiparameter imaging, is based mainly on chemical diversity of their chromophores. Recently we have reported, to our knowledge, a new green fluorescent protein WasCFP-the first fluorescent protein with a tryptophan-based chromophore in the anionic state. However, only a small portion of WasCFP(More)
We report an improved variant of mKeima, a monomeric long Stokes shift red fluorescent protein, hmKeima8.5. The increased intracellular brightness and large Stokes shift (∼180 nm) make it an excellent partner with teal fluorescent protein (mTFP1) for multiphoton, multicolor applications. Excitation of this pair by a single multiphoton excitation wavelength(More)
We report two cases of acute infectious supraglottitis in adults seen recently in our emergency department. The recent literature appropriate to the diagnosis and treatment of the entity is reviewed. In each of our cases the diagnosis was established with indirect laryngoscopy in the emergency department and appropriate therapy instituted without delay.(More)
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