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Acidic pH shock induces the expressions of a wide range of stress-response genes
BackgroundEnvironmental signals usually enhance secondary metabolite production in Streptomycetes by initiating complex signal transduction system. It is known that different sigma factors respond toExpand
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Diagnosis of HNF-1α mutations on a PNA zip-code microarray by single base extension
In the present study, we exploited the superior features of peptide nucleic acids (PNAs) to develop an efficient PNA zip-code microarray for the detection of hepatocyte nuclear factor-1α (HNF-1α)Expand
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Fluorescence-based assay formats and signal amplification strategies for DNA microarray analysis
TLDR
We broadly review fluorescence-based assay formats for nucleic acid detection on DNA microarrays with a focus on PCR-associated target preparation. Expand
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pH shock induces overexpression of regulatory and biosynthetic genes for actinorhodin productionin Streptomyces coelicolor A3(2)
Actinorhodin production is markedly enhanced when an acidic pH shock is applied to a surface-grown culture of Streptomyces coelicolor A3(2). For an in-depth study of this phenomenon, transcriptionalExpand
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Identification and characterization of a novel β-galactosidase from Victivallis vadensis ATCC BAA-548, an anaerobic fecal bacterium
Victivallis vadensis ATCC BAA-548 is a Gram-negative, anaerobic bacterium that was isolated from a human fecal sample. From the genomic sequence of V. vadensis, one gene was found to encode agarase;Expand
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Enhancement of geldanamycin production by pH shock in batch culture of Streptomyces hygroscopicus subsp. duamyceticus.
Various sequences of pH change were applied in a batch bioreactor to investigate pH shock effects on geldanamycin production by Streptomyces hygroscopicus subsp. duamyceticus JCM4427. In the controlExpand
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Effects of pH shock on the secretion system in Streptomyces coelicolor A3(2).
Effects of pH shock on the secretion system of S. coelicolor A3(2) have been investigated at a transcriptional level by using DNA microarrays. Actinorhodin secretion was observed to be highlyExpand
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Gene-expression analysis of acidic pH shock effects on two-component systems in Streptomyces coelicolor
In this study, transcriptional analyses by using reverse transcription-polymerase chain reaction (RT-PCR), DNA chip, and quantitative real time-PCR were performed to investigate effects of acidic pHExpand
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Heterologous expression of a putative K+/H+ antiporter of S. coelicolor A3(2) enhances K+, acidic-pH shock tolerances, and geldanamycin secretion.
Heterologous expression of a putative K+/H+ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to K+ stress,Expand
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