Jacques U . Baenziger

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Adducted thumb-clubfoot syndrome is an autosomal-recessive disorder characterized by typical facial appearance, wasted build, thin and translucent skin, congenital contractures of thumbs and feet, joint instability, facial clefting, and coagulopathy, as well as heart, kidney, or intestinal defects. We elucidated the molecular basis of the disease by using a(More)
The asparagine-linked oligosaccharides on the pituitary glycoprotein hormones lutropin (LH), follitropin (FSH), and thyrotropin (TSH) consist of a heterogeneous array of neutral, sulfated, sialylated, and sulfated/sialylated structures. In the accompanying paper (Green, E.D., and Baenziger, J.U. (1987) J. Biol. Chem. 262, 25-35), we elucidated the(More)
We have identified and characterized an N-acetylgalactosamine-4-O-sulfotransferase designated dermatan-4-sulfotransferase-1 (D4ST-1) (GenBank(TM) accession number AF401222) based on its homology to HNK-1 sulfotransferase. The cDNA predicts an open reading frame encoding a type II membrane protein of 376 amino acids with a 43-amino acid cytoplasmic domain(More)
Structural determinants of oligosaccharides affecting interaction with the saccharide-binding sites of two lectins, the agglutinin of M, 120,000 and toxin of 82, 60,000 (RCA* and RCA~I, respectively), isolated from Ricinus communis beans have been examined by performing binding studies with iodinated glycopeptides and their degradation products. Association(More)
The structures of the entire population of sialylated asparagine-linked oligosaccharides present on bovine fetuin were elucidated. Asparagine-linked oligosaccharides were released from fetuin with N-glycanase, radiolabeled by reduction with NaB[3H]4, and fractionated by anion-exchange high performance liquid chromatography (HPLC), ion-suppression amine(More)
Iodinated glycopeptides (50 to 200 x lo3 cpm/pmol) have been utilized to examine the structural determinants affecting interaction with the saccharide binding site of concanavalin A by Scatchard plot analyses of saturation curves. Glycopeptides with association constants in the range of 4.5 X 10’ M-’ and 25 X 10’ Mm1 are retained by concanavalin A-Sepharose(More)
High-gradient magnetic affinity chromatography (HIMAC) has been used to obtain highly enriched plasma membranes, free of intracellular membrane contaminants, from cultured Chinese hamster ovary (CHO) cells in yields of > or = 80%. Using this procedure we have characterized the transport of glucosylceramide (GlcCer) and the ganglioside GM3 to the plasma(More)
Luteinizing hormone (LH), follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH) from pituitary and chorionic gonadotropin (CG) from placenta are a family of closely related glycoproteins. Each hormone is a heterodimer, consisting of an alpha- and a beta-subunit. Within an animal species, the alpha-subunits of all four glyco-protein(More)
Expression of chimeras, composed of portions of two different glucose transporter isoforms (GLUT-1 and GLUT-4), in CHO cells had indicated that the cytoplasmic NH2 terminus of GLUT-4 contains important targeting information that mediates intracellular sequestration of this isoform (Piper, R. C., C. Tai, J. W. Slot, C. S. Hahn, C. M. Rice, H. Huang, D. E.(More)
We have elucidated the structures of the anionic asparagine-linked oligosaccharides present on the glycoprotein hormones lutropin (luteinizing hormone), follitropin (follicle-stimulating hormone), and thyrotropin (thyroid-stimulating hormone). Purified hormones, isolated from bovine, ovine, and human pituitaries, were digested with N-glycanase, and the(More)