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The production of polyhydroxybutyrate (PHB) by Alcaligenes eutrophus DSM 545 was studied in a synthetic medium with 3% glucose at pH 7.0 supplemented with several ammonium substrates and cane molasses. Growth was measured by dry cell weight, and the PHB content was measured by gas chromatography. The effects of ammonium sources such as sulfate, nitrate,(More)
BACKGROUND Iatrogenic paresthesia in the third division of the trigeminal nerve remains a complex clinical problem with major medicolegal implications. However, most lawsuits can be prevented through better planning of procedures and by obtaining informed consent. The purpose of this article is to present the authors" clinical experience over the past 12(More)
The aim of this study was to determine if probiotics reduce epithelial injury following exposure to Escherichia coli O157:H7 and E. coli O127:H6. The pretreatment of intestinal (T84) cells with lactic acid-producing bacteria reduced the pathogen-induced drop in transepithelial electrical resistance. These findings demonstrate that probiotics prevent(More)
The cryotolerance in frozen doughs and in water suspensions of bakers' yeast (Saccharomyces cerevisiae) previously grown under various industrial conditions was evaluated on a laboratory scale. Fed-batch cultures were very superior to batch cultures, and strong aeration enhanced cryoresistance in both cases for freezing rates of 1 to 56 degrees C min. Loss(More)
Kinetics of batch cultivation of four species of Bifidobacterium in milk were examined in detail. Bifidobacteria could grow well in milk inoculated with cultures prepared in a synthetic medium. Cessation of growth occurred, however, in pH-controlled batch cultures, although incomplete utilization of lactose was observed. Lactate and acetate accumulation(More)
This study investigated the physicochemical forces involving the adhesion of Listeria monocytogenes to surfaces. A total of 22 strains of L. monocytogenes were compared for relative surface hydrophobicity with the salt aggregation test. Cell surface charges and hydrophobicity of L. monocytogenes Scott A were also determined by electrophoretic mobility,(More)
The antimutagenic effects of whey, acetone extracts, and protein fractions isolated from milk that had been fermented by Lactobacillus helveticus L89 were investigated using the mutagen 4-nitroquinoline-N'-oxide in the Ames test (Salmonella typhimurium TA 100). Fermented milk significantly inhibited mutagenesis induced by 4-nitroquinoline-N'-oxide. However,(More)
Cheese whey ultrafiltrate (WU) was used as the carbon source for the production of lactic acid by batch fermentation with Lactobacillus helveticus strain milano. The fermentation was conducted in a 400 ml fermentor at an agitation rate of 200 rpm and under conditions of controlled temperature (42° C) and pH. In the whey ultrafiltrate-corn steep liquor(More)
In an effort to control the potential hazard of dairy product contamination by contact with processing surfaces, the efficiency of four commercial sanitizing agents was evaluated using the AOAC use-dilution method for their bactericidal activity at 4 and 20 degrees C against Listeria monocytogenes strain Scott-A attached on four types of surfaces (stainless(More)
The use of listeriaphages as a means of disinfecting contaminated stainless-steel and polypropylene surfaces was investigated. Surfaces artificially contaminated with L. monocytogenes 10401 and 8427 were sanitized with suspensions of listeriaphages (H387, H387-A, and 2671), all belonging to the Siphoviridae family. Phage suspensions at concentrations of up(More)