Jacob S. Hanker

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A new method for demonstrating cytochrome oxidase activity, based upon the oxidative polymerization of 3,3'-diaminobenzidine (DAB) to an osmiophilic reaction product, has improved the localization of this enzyme over methods based upon the Nadi reaction, in both the light and electron microscopes. The reaction product occurs in nondroplet form, which more(More)
3,3'-Diaminobenzidine (DAB), introduced by Graham & Karnovsky (J. Histochem. Cytochem. 1966, 14, 291 & J. exp. Med. 1966, 124, 1123) is one of the most widely employed reagents in histochemistry. It has been very useful in demonstrating the sites to which the exogenous ultrastructural tracer horseradish peroxidase (HRP) is transported in vertebrate tissues.(More)
Hydroperoxidase-positive Phi bodies and rods are much more prominent and prevalent than rods visualized with a Romanovsky-type stain (Auer rods) in immature leukocytes of patients with active acute myelogenous leukemia (AML). They are readily observed with the light microscope in peripheral blood or marrow films of AML patients stained to show their(More)
Recent studies in our laboratories have shown how microwave (MW) irradiation can accelerate a number of tissue-processing techniques, especially staining, to aid in the preparation of single specimens on glass microscope slides or coverslips for examination by light microscopy (and electron microscopy, if required) for diagnostic purposes. Techniques have(More)
Al though the in t roduct ion of fixation of tissue with osmium tetroxide by Palade (8) ushered in the modern era of electron microscopy of biological materials, a need for greater contrast and greater resolution in visualizing membranous structures has s t imulated the in t roduct ion of methods for staining with heavy metal salts, in order to enhance the(More)