Learn More
The pyruvate–malate shuttle is a metabolic cycle in pancreatic beta cells and is important for beta cell function. Cytosolic malic enzyme (ME) carries out an essential step in the shuttle by converting malate to pyruvate and generating NADPH. In rat islets the pyruvate–malate shuttle may regulate insulin secretion and it has been shown to play a critical(More)
Although islet transplantation in diabetes holds great promise, two or three donor pancreases are usually required to achieve normoglycaemia in human or rodent recipients. We investigated whether there were differences between fresh and cultured islets in terms of transplantation outcome. We also investigated the effects of normoglycaemia during engraftment(More)
Hyperglycaemia can impair beta cell function after islet transplantation. Appropriate glucose-induced insulin secretion is dependent on a unique expression pattern of genes. Here we examined the effects of diabetes on gene expression in transplanted islets. Streptozotocin-induced diabetic or control non-diabetic Lewis rats were transplanted under the kidney(More)
The novel antidiabetic agent BTS 67 582 (1,1-dimethyl-2-[2-(4-morpholinophenyl)]guanidine monofumarate) demonstrated a concentration-dependent stimulation of insulin release in perifused rat pancreatic islets. EC50 values of 7.7 microM and 6.3 microM were obtained for BTS 67 582 in the presence of 8 mM glucose, after islets were pre-equilibrated with 4 and(More)
Mucus constitutes a mesh-like hydrogel that pathogens must overcome to invade the underlying epithelium. The anti-microbial proteins, lactoferrin (LF) and lysozyme (LYS), are introduced during mucosal secretions and impact the microbiome by sequestering iron needed for bacterial growth and by lysing the cell wall. These proteins easily form ionic complexes(More)
  • 1