Jack Van’t Hof

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In synchronized root cells of Pisum sativum (cv. Alaska) the joining of nascent replicons is delayed until cells reach the S-G(2) boundary or early G(2) phase. To determine if the delayed ligation of nascent chains occurs at specific termination sites, we mapped the location of arrested forks in the ribosomal DNA (rDNA) repeats from cells in late S and G(2)(More)
The subject of this chapter is chromosomal DNA replication in higher plants. The discussion purposely is limited to results obtained with plants. References to viruses, plasmids, organelles, yeast, and other types of cells are few. Most of the pioneering work and insight on chromosomal DNA replication is traceable to research done with these organisms, but(More)
In cultured pea roots there is extrachromosomal DNA associated with cells that differentiate from the G(2) phase of the cell cycle that is absent from those that differentiate from the G(1) phase. We examined this extrachromosomal DNA by electron microscopy and found that it consisted of three types: (i) double-stranded linear molecules with single-stranded(More)
The use of synchronized populations of proliferative cells has been. advantageous for the investigation of events of the cell cycle (4, 5, 15) . Most investigations, however, have been carried out with procaryotic and eucaryotic single cells because dependable techniques for synchronization of cells in tissues were virtually unknown . It is therefore(More)
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