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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.
A novel protein purification system has been developed which enables purification of free recombinant proteins in a single chromatographic step. The system utilizes a modified protein splicingExpand
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Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells
Inheritance of epigenetic information encoded by cytosine DNA methylation patterns is crucial for mammalian cell survival, in large part through the activity of the maintenance DNA methyltransferaseExpand
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Semisynthesis of cytotoxic proteins using a modified protein splicing element.
Two cytotoxic proteins, bovine pancreatic ribonuclease A (RNase A), and a restriction endonuclease from Haemophilus parainfluenzae (HpaI), were produced using a novel semisynthetic approach thatExpand
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Automethylation of G9a and its implication in wider substrate specificity and HP1 binding
Methylation of lysine residues on histones participates in transcriptional gene regulation. Lysine 9 methylation of histone H3 is a transcriptional repression signal, mediated by a family of SETExpand
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Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation.
The determinants governing the self-catalyzed splicing and cleavage events by a mini-intein of 154 amino acids, derived from the dnaB gene of Synechocystis sp. were investigated. The residues at theExpand
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Protein trans-Splicing and Cyclization by a Naturally Split Intein from the dnaE Gene ofSynechocystis Species PCC6803*
A naturally occurring split intein from thednaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein) has been shown to mediate efficientin vivo and in vitro trans-splicing in a foreign proteinExpand
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The Cyclization and Polymerization of Bacterially Expressed Proteins Using Modified Self-splicing Inteins*
Mini-inteins derived fromSynechocystis sp. (Ssp DnaB intein) andMycobacterium xenopi (Mxe GyrA intein) that have been modified to cleave peptide bonds at their C and N termini, respectively, wereExpand
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Intervening sequences in an Archaea DNA polymerase gene.
The DNA polymerase gene from the Archaea Thermococcus litoralis has been cloned and expressed in Escherichia coli. It is split by two intervening sequences (IVSs) that form one continuous openExpand
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Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase.
The original cDNA sequence reported for the murine DNA methyltransferase (MTase) was not full length. Recently, additional cDNA sequences have been reported that lie upstream of the original andExpand
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Protein Splicing Involving the Saccharomyces cerevisiae VMA Intein
Protein splicing involves the excision of an internal protein segment, the intein, from a precursor protein and the concomitant ligation of the flanking N- and C-terminal regions. It occurs inExpand
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