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1. The effect of membrane potential on [Ca2+]i in rat megakaryocytes was studied using simultaneous whole-cell patch clamp and fura-2 fluorescence recordings. 2. Depolarization from -75 to 0 mV had no effect on [Ca2+]i in unstimulated cells, but evoked one or more spikes of Ca2+ increase (peak increase: 714 +/- 95 nM) during activation of metabotropic(More)
1. The effect of membrane potential (Vm) on ADP-evoked [Ca2+]i oscillations was investigated in rat megakaryocytes, a non-excitable cell type recently shown to exhibit depolarisation-evoked Ca2+ release from intracellular stores during metabotropic purinoceptor stimulation. 2. Hyperpolarising voltage steps caused a transient fall in [Ca2+]i and either(More)
1. A combination of conventional whole-cell patch clamp recordings and fura-2 fluorescence photometry was used to study the membrane currents during oscillations of intracellular Ca2+ concentration ([Ca2+]i) in single rat megakaryocytes. 2. At a holding potential of -60 mV, in NaCl external saline and KCl internal saline with low levels of Ca2+ buffering,(More)
1. We have developed conditions that permit long duration recordings of [Ca2+]i in single, isolated human platelets and studied the reversibility of Ca2+i spiking following activation by physiological and artificial stimuli. 2. Fura-2-loaded platelets were immobilized at the tip of a saline-filled glass pipette using gentle suction. 'Contact' activation of(More)
To compete successfully in today's challenging business environment, companies need to focus on SCM practices that have great impact on enhancing their performances. This research conceptualizes the effects of SCM practices (SSP, IS, and POS) and tests the relationships between SCM practices and OP in consumer goods manufacturing industry at Pakistan. A(More)
1. We have used the imidazoline derivative [3H]-idazoxan to define alpha 2-adrenoceptors and non-adrenoceptor, imidazoline binding sites in cerebral cortex membranes of calf, mouse, rat, guinea-pig and man. 2. Competition experiments using the selective alpha-adrenoceptor drugs, rauwolscine and corynanthine, indicated that [3H]-idazoxan bound to a single(More)
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