JOAN E. LUSK

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Mutants in three genes affecting two Mg2+ transport systems are described. System I, for which Co2+, Mn2+, and Mg2+ are substrates, is inactive in corA mutants corB mutants express system I after growth on high (10 mM) Mg2+ but not low (0.1 mM) Mg2+. Both corA and corB mutants are resistant to Co2+ or Mn2+. corA mutants are sensitive to CA2+. Transport(More)
Conditions permitting survival (colony formation) of E. coli after treatment with colicin K have been found. Survival required K+ and Mg2+ at concentrations high enough to replace the intracellular ions lost from colicintreated cells. Either glucose minimal medium or broth could support survival. Survival was still observed after colicin-promoted efflux of(More)
Cavernous hemangioma of the optic disc and retina is a vascular hamartoma composed of clusters of thin-walled saccular aneurysms partially filled with dark venous blood. It is now considered a neuro-oculo-cutaneous syndrome whose mode of inheritance is probably autosomal dominant. This report describes a cavernous hemangioma of the optic disc in a patient(More)
A mutant of Escherichia coli has been isolated, the growth of which is inhibited by low concentrations (1 mm) of NaCl. High levels of magnesium, calcium, or strontium in the medium permit growth in the presence of sodium. The metal content of the inhibited mutant is normal, but the strain is unable to tolerate levels of sodium to which the wild type is(More)
The growth of cells of Escherichia coli has been studied in media with varying amounts of magnesium. Experiments in which the content of magnesium in the medium and in the cell have been directly measured reveal that the growth rate of magnesium-limited wild type cells is half-maximal at extracelhrlar concentrations of 1 to 3 X low6 M. Mutants have been(More)
The energy-dependent exchange of intracellular Mg(2+) with extracellular Mg(2+) or Co(2+) is inhibited by colicin E1 and, less strongly, by colicin K. Treatment with either colicin causes a net loss of intracellular Mg(2+). This loss begins immediately in cells treated with colicin E1, but in colicin K-treated cells the onset of Mg(2+) loss is delayed 1 to(More)
Since it is impossible to sterilize the surgical field in ocular surgery, postoperative endophthalmitis remains a serious complication of intraocular surgery. The surgeon should take all reasonable precautions to avoid this disaster. We believe that this includes isolating the skin and eyelids from the operative field with the incise drape.
The growth of cells of Escherichia coli has been studied in media with varying amounts of magnesium. Experiments in which the content of magnesium in the medium and in the cell have been directly measured reveal that the growth rate of magnesium-limited wild type cells is half-maximal at extracelhrlar concentrations of 1 to 3 X low6 M. Mutants have been(More)
1. A mutant lacking both detergent-resistant and detergent-sensitive phospholipase A activities is fully sensitive to colicin K. 2. In the absence of cellular phospholipases A, colicin K does not promote hydrolysis of phosphatidylethanolamine. 3. Cells of the colicin-treated mutant lacking lysophosphatidylethanolamine are as abnormally permeable to Co-2+(More)