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Modern biotechnologies are used extensively in the animal breeding industry today. Therefore, it is essential that sperm handling procedures do not modulate the normal physiological mechanisms occurring in the female reproductive tract. In this paper, the different selection mechanisms occurring in vivo are described briefly, together with their relevance(More)
Sperm quality in insemination doses is known to affect pregnancy rates following artificial insemination (AI) in a number of animal species. Furthermore, biotechnologies such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) require the spermatozoa to be removed from seminal plasma, which contains inhibitory substances, cellular(More)
On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter(More)
Colloid centrifugation can be used to separate heterogeneous populations of cells particularly semen samples, which typically contain mixtures of spermatozoa at different stage of maturity together with dead and dying spermatozoa and also non-sperm cells. Colloids can be used to enrich the population of spermatozoa that are viable and functional; these are(More)
Single-Layer centrifugation has been used to improve the quality of sperm samples in several species. However, where stallion or boar semen is to be used for AI, larger volumes of semen have to be processed than for other species, thus limiting the effectiveness of the original technique. The objective of the present study was to scale up the SLC method for(More)
The osmolarity and density of colloids used to prepare spermatozoa for assisted reproduction may affect sperm quality in the resultant preparation. In this study, two osmolarities of Androcoll-E for single-layer or density gradient centrifugation of stallion spermatozoa were compared: "normal" (320 mOsm) or "high" (345 mOsm). Mean yields for the two(More)
Visual motility analysis is the basis for routine quality evaluation of stallion semen, although its prognostic value for fertilizing ability is considered low. The present study evaluated the ability of a novel computer-assisted motility analyzer (QualiSpermTM) to determine the motility and velocity of ejaculated, extended stallion spermatozoa (collected(More)
The effect on sperm motility and chromatin integrity of adding homologous or heterologous equine seminal plasma (SP) to fresh stallion spermatozoa selected by single layer centrifugation (SLC) was studied. No statistical difference in mean progressive motility was seen after adding SP at time 0 h, although there were differences for individual stallions.(More)
The susceptibility of ejaculated stallion spermatozoa to lipid peroxidation (LPO) was studied in ejaculates (n=4) from each of three stallions before and after a single layer centrifugation (SLC) through AndrocollTM-E. Each ejaculate was split, and aliquots extended with one of three different commercial extenders: INRA 96, KENNEY ́s or Equipro extenders(More)
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