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Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight.
TLDR
The current study confirms the important role of DGAT in regulating the quantity of seed triacylglycerols and the sink size in developing seeds and shows for the first time that seed-specific over-expression of the DGAT cDNA in wild-type Arabidopsis enhances oil deposition and average seed weight, which are correlated with DGAT transcript levels. Expand
The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene.
TLDR
The TAG1 cDNA was over-expressed in yeast, and its activity as a microsomal DGAT confirmed, and the insertion mutation in the TAG1 gene in mutant AS11 results in its altered lipid phenotype. Expand
Alteration of Seed Fatty Acid Composition by an Ethyl Methanesulfonate-Induced Mutation in Arabidopsis thaliana Affecting Diacylglycerol Acyltransferase Activity
TLDR
It is demonstrated that changes to triacylglycerol biosynthesis can result in dramatic changes in fatty acid composition and, in particular, in the accumulation of VLCFAs in seed storage lipids. Expand
Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene.
TLDR
The yeast sn-2 acyltransferase has been shown to encode a protein that can exhibit lysophosphatidic acid acyl transferase activity and that can be used to change total fatty acid content and composition as well as to alter the stereospecific acyl distribution of fatty acids in seed TAGs. Expand
Tracing the Transcriptomic Changes in Synthetic Trigenomic allohexaploids of Brassica Using an RNA-Seq Approach
TLDR
There were more significant differences in gene expression between the allohexaploid and its paternal parent than between it and its maternal parent, possibly partly because of cytoplasmic and maternal effects. Expand
Synthesis of a Brassica trigenomic allohexaploid (B. carinata × B. rapa) de novo and its stability in subsequent generations
TLDR
The present investigation was aimed at synthesising the first known chromosomally stable hexaploid Brassica with the genome constitution AABBCC, and the appearance of aneuploid plants in each generation could be detected having various chromosomal abnormalities at meiosis. Expand
Induction of Lipid and Oleosin Biosynthesis by (+)-Abscisic Acid and Its Metabolites in Microspore-Derived Embryos of Brassica napus L.cv Reston (Biological Responses in the Presence of
TLDR
To the authors' knowledge, this is the first report of the biological activity of 8[prime]-OH ABA and of stimulatory effects of (+)-ABA and 8[ prime]- OH ABA on lipid and oleosin biosynthesis. Expand
Association mapping of seed oil content in Brassica napus and comparison with quantitative trait loci identified from linkage mapping.
TLDR
Relatively rich genetic diversity, but a higher level of linkage disequilibrium was observed in the new-type B. napus as compared with the traditional B.Napus, and more than half of the genetic loci, to which the associated markers corresponded, were located within the quantitative trait loci intervals identified previously in linkage mapping experiments, which demonstrated the power of association mapping in B. Napus. Expand
The plastidic phosphoglucomutase from Arabidopsis. A reversible enzyme reaction with an important role in metabolic control.
TLDR
The results indicate that plastidic PGM is an important factor affecting carbon flux in triacylglycerol accumulation in oilseed plants, most likely through its essential role in starch synthesis. Expand
A Dynamic and Complex Network Regulates the Heterosis of Yield-Correlated Traits in Rapeseed (Brassica napus L.)
TLDR
A reconstructed F2 population was produced by random intercross among 202 lines of a double haploid population in rapeseed, indicating heterozygosity was not always advantageous for heterosis/hybrid performance and the implications on evolution and crop breeding are discussed. Expand
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