An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database
Histone H3 Methylation by Set2 Directs Deacetylation of Coding Regions by Rpd3S to Suppress Spurious Intragenic Transcription
Large-scale analysis of the yeast proteome by multidimensional protein identification technology
MudPIT was applied to the proteome of the Saccharomyces cerevisiae strain BJ5460 grown to mid-log phase and yielded the largest proteome analysis to date, identifying 131 proteins with three or more predicted transmembrane domains which allowed us to map the soluble domains of many of the integral membrane proteins.
Wnt proteins are lipid-modified and can act as stem cell growth factors
This work isolated active Wnt molecules, including the product of the mouse Wnt3a gene, and found the proteins to be palmitoylated on a conserved cysteine, indicating that the lipid is important for signalling.
Microglia Promote Learning-Dependent Synapse Formation through Brain-Derived Neurotrophic Factor
DTASelect and Contrast: tools for assembling and comparing protein identifications from shotgun proteomics.
DTASelect, a new software package, assembles SEQUEST identifications and highlights the most significant matches, and the accompanying Contrast tool compares DTASelect results from multiple experiments to improve the speed and precision of proteomic data analysis.
A role for the P-body component GW182 in microRNA function
- Jidong Liu, F. Rivas, J. Wohlschlegel, J. Yates, R. Parker, G. Hannon
- BiologyNature Cell Biology
- 13 November 2005
The results support a functional link between cytoplasmic P-bodies and the ability of a microRNA to repress expression of a target mRNA.
Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database.
The approach described in this paper provides a convenient method to match the nascent tandem mass spectra of modified peptides to sequences in a protein database and thereby identify previously unknown sites of modification.
The hMre11/hRad50 Protein Complex and Nijmegen Breakage Syndrome: Linkage of Double-Strand Break Repair to the Cellular DNA Damage Response