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An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database
TLDR
The approach described in this manuscript provides a convenient method to interpret tandem mass spectra with known sequences in a protein database. Expand
Histone H3 Methylation by Set2 Directs Deacetylation of Coding Regions by Rpd3S to Suppress Spurious Intragenic Transcription
TLDR
Data indicate that Pol II-associated Set2 methylates H3 providing a transcriptional memory which signals for deacetylation of ORFs by Rpd3S, which erases transcription elongation-associated acetylation to suppress intragenic transcription initiation. Expand
Large-scale analysis of the yeast proteome by multidimensional protein identification technology
TLDR
MudPIT was applied to the proteome of the Saccharomyces cerevisiae strain BJ5460 grown to mid-log phase and yielded the largest proteome analysis to date, identifying 131 proteins with three or more predicted transmembrane domains which allowed us to map the soluble domains of many of the integral membrane proteins. Expand
Wnt proteins are lipid-modified and can act as stem cell growth factors
TLDR
This work isolated active Wnt molecules, including the product of the mouse Wnt3a gene, and found the proteins to be palmitoylated on a conserved cysteine, indicating that the lipid is important for signalling. Expand
Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database.
TLDR
The approach described in this paper provides a convenient method to match the nascent tandem mass spectra of modified peptides to sequences in a protein database and thereby identify previously unknown sites of modification. Expand
DTASelect and Contrast: tools for assembling and comparing protein identifications from shotgun proteomics.
TLDR
DTASelect, a new software package, assembles SEQUEST identifications and highlights the most significant matches, and the accompanying Contrast tool compares DTASelect results from multiple experiments to improve the speed and precision of proteomic data analysis. Expand
Acetylation by Tip60 Is Required for Selective Histone Variant Exchange at DNA Lesions
TLDR
It is demonstrated that the Drosophila Tip60 (dTip60) chromatin-remodeling complex acetylates nucleosomal phospho-H2Av and exchanges it with an unmodified H2Av, revealing a previously unknown mechanism for selective histone exchange that uses the concerted action of two distinct Chromatin-Remodeling enzymes within the same multiprotein complex. Expand
The hMre11/hRad50 Protein Complex and Nijmegen Breakage Syndrome: Linkage of Double-Strand Break Repair to the Cellular DNA Damage Response
TLDR
The isolated p95 gene encoding p95, a member of the hMre11/hRad50 double-strand break repair complex, reveals a direct molecular link between DSB repair and cell cycle checkpoint functions in NBS. Expand
A role for the P-body component GW182 in microRNA function
TLDR
The results support a functional link between cytoplasmic P-bodies and the ability of a microRNA to repress expression of a target mRNA. Expand
The human CENP-A centromeric nucleosome-associated complex
TLDR
It is shown that CENP-A nucleosomes directly recruit a proximal CEN parenthood-associated nucleosome associated complex (NAC) comprised of three new human centromere proteins (CENp-M, CenP-N and CENT-T), along with C ENP-U(50), CEN P-C and C ENp-H. Expand
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