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Localizing the NADP+ binding site on the MurB enzyme by NMR
NMR resonances of backbone nuclei have been assigned for >95% of the residues in oxidized, substrate-free, perdeuterated 13C/15N-labelled MurB (347 residues). A novel approach utilizing minimum…
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Correlation of High-Throughput Pregnane X Receptor (PXR) Transactivation and Binding Assays
TLDR
There were discrepancies with some compounds showing high binding affinity in the binding assay translated into low transactivation, the most likely cause for these discrepancies was an agonist-dependent relationship between binding affinity and transactivation response.
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Steady-state kinetic mechanism of Escherichia coli UDP-N-acetylenolpyruvylglucosamine reductase.
TLDR
The data suggest that NADPH and UNAGEP share a subsite that prevents both molecules from binding at once, which is consistent with a ping pong bi bi double competitive substrate inhibition mechanism.
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Involvement of DPP‐IV catalytic residues in enzyme–saxagliptin complex formation
TLDR
The structural and binding data presented here strongly support a histidine‐assisted covalent bond formation between S630 hydroxyl oxygen and the nitrile group of saxagliptin.
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Molecular Basis for Increased Susceptibility of Isolates with Atazanavir Resistance-Conferring Substitution I50L to Other Protease Inhibitors
TLDR
The results of this study provide a molecular understanding of the novel hypersusceptibility of atazanavir-resistant I50L/A71V-containing clinical isolates to other currently approved PIs.
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Characterization of NADP+ binding to perdeuterated MurB: backbone atom NMR assignments and chemical-shift changes.
TLDR
The NMR data indicate that NADP+ and UNAGEP utilize the same binding pocket and, furthermore, that the binding of NADP- induces structural changes in MurB, consistent with MurB having a flexible active site.
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Evaluation of the kinetic mechanism of Escherichia coli uridine diphosphate-N-acetylmuramate:L-alanine ligase.
TLDR
The production of acid-labile phosphate as a function of time is characterized by a burst phase followed by a slower linear phase with the rate close to k(cat) in the presence of all three substrates, consistent with L-alanine being the third substrate to bind in a sequential mechanism involving a putative acyl-phosphate intermediate.
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Aroylguanidine-based factor Xa inhibitors: the discovery of BMS-344577.
  • Yan Shi, Chi Li, P. Stein
  • Chemistry, Biology
    Bioorganic & medicinal chemistry letters
  • 15 December 2009
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Decrypting the Biochemical Function of an Essential Gene from Streptococcus pneumoniae Using ThermoFluor® Technology*
TLDR
The utility of a generic affinity-based ligand binding technology in decrypting possible biological functions of a protein is demonstrated, an approach that is both independent of and complementary to existing genomic and proteomic technologies.
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Spectroscopic properties of Escherichia coli UDP-N-acetylenolpyruvylglucosamine reductase.
Purified uridine diphosphate N-acetylenolpyruvylglucosamine reductase (E.C. 1.1.1.158) was analyzed by circular dichroism (CD) and UV-visible spectroscopy to establish the spectral properties of its…
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