A perspective on the context and evolutionary significance of hybridization during speciation is offered, highlighting issues of current interest and debate and suggesting that the Dobzhansky–Muller model of hybrid incompatibilities requires a broader interpretation.
This study provides a roadmap to the emerging field of speciation genomics by showing that the genomic landscape of species differentiation is highly heterogeneous with approximately 50 ‘divergence islands’ showing up to 50-fold higher sequence divergence than the genomic background.
Characterization of genomic differentiation in a classic example of hybridization between all-black carrion crows and gray-coated hooded crows identified genome-wide introgression extending far beyond the morphological hybrid zone, indicating localized genomic selection can cause marked heterogeneity in introgressive landscapes while maintaining phenotypic divergence.
This meta-analysis showed that ecologically induced genetic divergence is pervasive across time-scales and taxa, and largely independent of the choice of molecular marker.
This Review explores methodological trends in speciation genomic studies, highlights the difficulty in separating processes related to speciation from those emerging from genome-wide properties that are not related to reproductive isolation, and provides a set of suggestions for future work in this area.
It is recommended that RAD‐seq studies employ reference‐based approaches to a closely related genome, and due to the high stochasticity associated with the pipeline advocate the use of multiple pipelines to ensure robust population genetic and demographic inferences.
This tutorial is targeted at scientists with a background in conservation genetics, but more generally, provides useful practical guidance for researchers engaging in whole‐genome sequencing projects.
This article gives a broad overview and provides practical guidance for the many steps involved in a typical RNA‐seq work flow from sampling, to RNA extraction, library preparation and data analysis.