Fluorescence method for measuring the kinetics of fusion between biological membranes.
- D. Hoekstra, T. de Boer, K. Klappe, J. Wilschut
- Biology, ChemistryBiochemistry
- 20 November 1984
By use of this method, the kinetics of fusion between Sendai virus and erythrocyte ghosts and virus-induced fusion of ghosts were readily revealed and were not related to processes other than fusion.
Dengue virus life cycle: viral and host factors modulating infectivity
- I. Rodenhuis-Zybert, J. Wilschut, J. Smit
- MedicineCellular and Molecular Life Sciences
- 6 April 2010
An overview of the infectious life cycle of DENV is given and the viral and host factors that are important in controlling DENV infection are discussed.
Dissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells
- H. M. van der Schaar, Michael J Rust, J. Smit
- BiologyPLoS Pathogens
- 1 December 2008
Simultaneous tracking of DENV particles and various endocytic markers revealed that DENV enters cells exclusively via clathrin-mediated endocytosis, and fusion of the viral membrane with the endosomal membrane was primarily detected in late endOSomal compartments.
Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein
- J. Wahlberg, R. Bron, J. Wilschut, H. Garoff
- BiologyJournal of Virology
- 1 December 1992
Support is given for a model in which the E1 trimeric structure is involved in the SFV-mediated fusion reaction, and it is shown that the new oligomer is represented by an E1 trimmeder.
Immature Dengue Virus: A Veiled Pathogen?
- I. Rodenhuis-Zybert, H. M. van der Schaar, J. Smit
- Biology, MedicinePLoS Pathogens
- 1 January 2010
It is demonstrated that prM antibodies facilitate efficient binding and cell entry of immature particles into Fc-receptor-expressing cells and enzymatic activity of furin is critical to render the internalized immature virus infectious.
Membrane fusion of Semliki Forest virus in a model system: correlation between fusion kinetics and structural changes in the envelope glycoprotein.
- R. Bron, J. Wahlberg, H. Garoff, J. Wilschut
- BiologyEMBO Journal
- 1 February 1993
A kinetic analysis of low‐pH‐induced fusion of Semliki Forest virus with cholesterol‐containing unilamellar lipid vesicles (liposomes) suggests that the E1 homotrimeric structure represents the fusion‐active conformation of the SFV spike, the actual fusion complex possibly involving a higher order oligomer of E1 trimers.
The virosome concept for influenza vaccines.
- A. Huckriede, L. Bungener, J. Wilschut
- BiologyVaccine
- 8 July 2005
Interaction of the HIV-1 fusion peptide with phospholipid vesicles: different structural requirements for fusion and leakage.
- J. L. Nieva, S. Nir, A. Muga, F. Goñi, J. Wilschut
- Biology, ChemistryBiochemistry
- 22 March 1994
This paper presents a study on the membrane fusion activity of a 23-residue synthetic peptide, representing the N-terminus of gp41 of the human immunodeficiency virus type I (HIV-1; LAV1a strain), in…
Membrane fusion activity of the influenza virus hemagglutinin: interaction of HA2 N-terminal peptides with phospholipid vesicles.
- M. Rafalski, A. Ortiz, J. Wilschut
- Biology, ChemistryBiochemistry
- 22 October 1991
Circular dichroism spectroscopy measurements of peptides in POPC SUV showed that the conformations of all three peptides are sensitive to pH, but only the wt and E4 peptides became predominantly alpha-helical at acid pH.
Development of Stable Influenza Vaccine Powder Formulations: Challenges and Possibilities
- Jean‐Pierre Amorij, A. Huckriede, J. Wilschut, H. Frijlink, W. Hinrichs
- BiologyPharmaceutical Research
- 13 March 2008
This review represents the current status of dry stable inactivated influenza vaccine development and the challenges for ultimate market introduction of a stable and effective dry-powder influenza vaccine.
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