A Role for Caveolin and the Urokinase Receptor in Integrin-mediated Adhesion and Signaling
- Ying-Ying Wei, Xiuwei H Yang, Qiumei Liu, J. Wilkins, H. Chapman
- Biology, ChemistryJournal of Cell Biology
- 22 March 1999
It is concluded that ligand-induced signaling necessary for normal β1 integrin function requires caveolin and is regulated by uPAR, and Caveolin and uPAR may operate within adhesion sites to organize kinase-rich lipid domains in proximity to integrins, promoting efficient signal transduction.
An Improved Model for Prediction of Retention Times of Tryptic Peptides in Ion Pair Reversed-phase HPLC
The proposed model is based on the measurement of the retention times of 346 tryptic peptides in the 560- to 4,000-Da mass range, derived from a mixture of 17 protein digests, and can be used for accurate prediction of retention times fortryptic peptide on reversed-phase columns of different sizes with a linear water-ACN gradient and with TFA as the ion-pairing modifier.
Requirement of Podocalyxin in TGF-Beta Induced Epithelial Mesenchymal Transition
The expression of podocalyxin (PODXL), a member of the CD34 family, is markedly increased during TGF-β induced EMT and PODXL is enriched on the leading edges of migrating A549 cells.
The role of angiotensin converting enzyme 2 in the generation of angiotensin 1-7 by rat proximal tubules.
- Ningjun Li, J. Zimpelmann, K. Cheng, J. Wilkins, K. Burns
- BiologyAJP - Renal Physiology
- 1 February 2005
The results indicate that ACE2 is widely expressed in rat nephron segments and contributes to the production of ANG 1-7 from ANG I in PST, which represents an important component of the proximal tubular renin-ANG system.
CD40 ligand binds to alpha5beta1 integrin and triggers cell signaling.
Evidence is provided that soluble CD40L (sCD40L) binds to cells of the undifferentiated human monocytic U937 cell line in a CD40- and alphaIIbbeta3-independent manner and the existence of novel CD40 L-dependent pathways of physiological relevance for cells expressing multiple receptors (CD40, alpha5beta1, and alpha IIbbeta 3) for CD 40L is documented.
Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression
Relative expression profiles demonstrate which proteins are likely utilized in carbohydrate utilization and end-product synthesis and suggest that H2 synthesis occurs via bifurcating hydrogenases while ethanol synthesis is predominantly catalyzed by a bifunctional aldehyde/alcohol dehydrogenase.
Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry.
- S. Schaub, J. Wilkins, T. Weiler, K. Sangster, D. Rush, P. Nickerson
- Medicine, BiologyKidney International
SELDI-TOF-MS offers a unique platform for high throughput urine protein profiling; however, standardization of analysis conditions is critical, and both extrinsic and intrinsic factors must be taken into account for accurate data interpretation.
Proteomic-based detection of urine proteins associated with acute renal allograft rejection.
Proteomic technology together with stringent definition of patient groups can detect urine proteins associated with acute renal allograft rejection and Identification of these proteins may prove useful as non-invasive diagnostic markers for rejection and the development of novel therapeutic agents.
Quantitative Proteomic Analyses of Influenza Virus-Infected Cultured Human Lung Cells
Gene ontology and pathway analyses indicated differentially regulated proteins and included those involved in host cell immunity and antigen presentation, cell adhesion, metabolism, protein function, signal transduction, and transcription pathways.
Deamidation of -Asn-Gly- sequences during sample preparation for proteomics: Consequences for MALDI and HPLC-MALDI analysis.
- O. Krokhin, Mihaela Antonovici, W. Ens, J. Wilkins, K. Standing
- BiologyAnalytical Chemistry
- 21 July 2006
It is found that peptides containing -Asn-Gly- sequences typically show approximately 70-80% degree of deamidation after standard overnight tryptic digestion at 37 degrees C, and analysis of a large pool of peptide retention data shows that the -betaAsp-/-Asn/ -AsP- retention order is normally observed under the above conditions.