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The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.
The most suitable dyes tested were fluorescein diacetate for viable cells and phenosafranine for dead cells and these dyes stained specifically whether the cells were from cultures of different ages, of different species or if the cells had been treated in various, often toxic, ways. Expand
Miscanthus: A Promising Biomass Crop
M. × giganteus has great promise as an economically and ecologically viable biomass crop; however, there are still challenges to widespread commercial development. Expand
Chemical components with health implications in wild and cultivated Mexican common bean seeds (Phaseolus vulgaris L.).
The impact on health of common bean seed is affected by dietary burden, specific compounds content, and processing, and on the other hand, germinated bean seed or beans sprouts may be sources of antioxidants and phytoestrogens. Expand
High-efficiency induction of soybean hairy roots and propagation of the soybean cyst nematode
The soybean cyst nematode could complete its entire life cycle in transformed soybean hairy-root cultures expressing GFP, and this system should be ideal for testing genes that might impart resistance to soybean Cyst Nematode. Expand
Effect of temperature and soil moisture status during seed development on soybean seed isoflavone concentration and composition
The overall results show that low temperatures and high soil moisture conditions produced the highest seed isoflavone concentrations with changes in temperature having the larger effect. Expand
Characterization and purification of a phytotoxin produced by Fusarium solani, the causal agent of soybean sudden death syndrome
A phytotoxic polypeptide identified in culture filtrates of Fusarium solani, the causal agent of soybean sudden death syndrome, was heat unstable, negatively charged, absorbed by 10% charcoal, andExpand
Photoautotrophic growth of soybean cells in suspension culture: I. Establishment of photoautotrophic cultures.
Highly chlorophyllous photomixotrophic callus was visually selected from callus originating from soybean (Glycine max (L.) Merr. var. Corsoy) cotyledon. Suspension cultures initiated from this callusExpand
Prevention of pink-pigmented methylotrophic bacteria (Methylohacterium mesophilicum) contamination of plant tissue cultures
While PPFMs are present on the surfaces of most plant tissues, surface disinfestation alone can effectively remove them so that uncontaminated tissue cultures can be initiated in most cases. Expand
Polyphenols in wild and weedy Mexican common beans (Phaseolus vulgaris L.).
Some wild and weedy beans are good sources of phenolic compounds for use in breeding programs focused on nutrition and health, and the wide variation observed in polyphenolic contents was more related to their genotype than to the color factor. Expand
The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes
Tissue-culture techniques are now available to obtain callus cultures capable of plant regeneration from immature embryos of most maize genotypes, and plant regeneration was noted in many commercially important inbreds. Expand