Phytochemistry of the genus Piper
LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition
LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA.
The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.
Potent and nontoxic antisense oligonucleotides containing locked nucleic acids.
- C. Wahlestedt, P. Salmi, J. Wengel
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 9 May 2000
It is demonstrated here that a high-affinity DNA analog, locked nucleic acid (LNA), confers several desired properties to antisense agents, and LNA/DNA copolymers exhibited potent antisense activity on assay systems as disparate as a G-protein-coupled receptor in living rat brain and an Escherichia coli reporter gene.
LNA: a versatile tool for therapeutics and genomics.
Synthetic Genetic Polymers Capable of Heredity and Evolution
It is shown that genetic information can be stored in and recovered from six alternative genetic polymers based on simple nucleic acid architectures not found in nature [xeno-nucleic acids (XNAs)] and selected XNA aptamers, which bind their targets with high affinity and specificity, demonstrating that beyond heredity, specific XNAs have the capacity for Darwinian evolution and folding into defined structures.
NMR studies of fully modified locked nucleic acid (LNA) hybrids: solution structure of an LNA:RNA hybrid and characterization of an LNA:DNA hybrid.
- K. Nielsen, J. Rasmussen, Ravindra Kumar, J. Wengel, J. Jacobsen, M. Petersen
- Biology, ChemistryBioconjugate chemistry
- 4 May 2004
The high-resolution structure reveals that the LNA:RNA hybrid adopts an almost canonical A-type duplex morphology, which shows that fully modified LNA oligomers can hybridize with complementary RNA and form duplexes within the Watson-Crick framework.
Locked nucleic acid (LNA) recognition of RNA: NMR solution structures of LNA:RNA hybrids.
- M. Petersen, K. Bondensgaard, J. Wengel, J. Jacobsen
- Biology, ChemistryJournal of the American Chemical Society
- 4 May 2002
It is suggested that the change in electronic density at the brim of the minor groove, introduced by the LNA modification, is causing an alteration of the pseudorotational profile of the 3'-flanking nucleotide, thus shifting this sugar equilibrium toward N-type conformation.
The effect of chemical modification and nanoparticle formulation on stability and biodistribution of siRNA in mice.
Improvements to siRNA stability and pharmacokinetics are highlighted, key determinants for development of clinically relevant RNAi therapeutics.
LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition
A novel class of nucleic acid analogues, termed LNA (locked nucleic acids), is introduced. Following the Watson–Crick base pairing rules, LNA forms duplexes with complementary DNA and RNA with…