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Phytochemistry of the genus Piper
Abstract The secondary metabolites isolated from Piper species for the period 1907 to June 1996 have been reviewed. Nearly six hundred chemical constituents belonging to different classes ofExpand
LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition
Abstract LNA (Locked Nucleic Acids), consisting of 2′- O ,4′- C -methylene bicyclonucleoside monomers, is efficiently synthesized and its nucleic acid recognition potential evaluated for sixExpand
LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA.
The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein. Expand
Potent and nontoxic antisense oligonucleotides containing locked nucleic acids.
It is demonstrated here that a high-affinity DNA analog, locked nucleic acid (LNA), confers several desired properties to antisense agents, and LNA/DNA copolymers exhibited potent antisense activity on assay systems as disparate as a G-protein-coupled receptor in living rat brain and an Escherichia coli reporter gene. Expand
LNA: a versatile tool for therapeutics and genomics.
Several reports have revealed LNA as a most promising molecule for the development of oligonucleotide-based therapeutics, including high capturing efficiencies and unambiguous scoring of single-nucleotide polymorphisms. Expand
Synthetic Genetic Polymers Capable of Heredity and Evolution
It is shown that genetic information can be stored in and recovered from six alternative genetic polymers based on simple nucleic acid architectures not found in nature [xeno-nucleic acids (XNAs)] and selected XNA aptamers, which bind their targets with high affinity and specificity, demonstrating that beyond heredity, specific XNAs have the capacity for Darwinian evolution and folding into defined structures. Expand
Locked nucleic acid (LNA) recognition of RNA: NMR solution structures of LNA:RNA hybrids.
It is suggested that the change in electronic density at the brim of the minor groove, introduced by the LNA modification, is causing an alteration of the pseudorotational profile of the 3'-flanking nucleotide, thus shifting this sugar equilibrium toward N-type conformation. Expand
NMR studies of fully modified locked nucleic acid (LNA) hybrids: solution structure of an LNA:RNA hybrid and characterization of an LNA:DNA hybrid.
The high-resolution structure reveals that the LNA:RNA hybrid adopts an almost canonical A-type duplex morphology, which shows that fully modified LNA oligomers can hybridize with complementary RNA and form duplexes within the Watson-Crick framework. Expand
Improved thrombin binding aptamer by incorporation of a single unlocked nucleic acid monomer
A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity andExpand
Improved silencing properties using small internally segmented interfering RNAs
A novel siRNA design composed of an intact antisense strand complemented with two shorter 10–12 nt sense strands, which suggests that the sisiRNA design has a clear potential of improving the pharmacokinetic properties of siRNA in vivo. Expand