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A Phosphorylated Pseudokinase Complex Controls Cell Wall Synthesis in Mycobacteria
Structure-function studies in mycobacteria reveal how a Ser-Thr protein kinase and pseudokinase work together to regulate the synthesis of the bacterial cell wall. The Bacterial Cell WallExpand
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  • Open Access
The LuxR family protein SpnR functions as a negative regulator of N‐acylhomoserine lactone‐dependent quorum sensing in Serratia marcescens
Serratia marcescens SS‐1 produces at least four N ‐acylhomoserine lactones (AHLs) which were identified using high‐resolution mass spectrometry and chemical synthesis, as N‐ (3‐oxohexanoyl)Expand
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Identification of a Mycobacterium tuberculosis gene that enhances mycobacterial survival in macrophages.
Intracellular survival plays a central role in the pathogenesis of Mycobacterium tuberculosis. To identify M. tuberculosis genes required for intracellular survival within macrophages, an M.Expand
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Depletion of antibiotic targets has widely varying effects on growth
It is often assumed that antibiotics act on the most vulnerable cellular targets, particularly those that require limited inhibition to block growth. To evaluate this assumption, we developed aExpand
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Post-Translational Regulation via Clp Protease Is Critical for Survival of Mycobacterium tuberculosis
Unlike most bacterial species, Mycobacterium tuberculosis depends on the Clp proteolysis system for survival even in in vitro conditions. We hypothesized that Clp is required for the physiologicExpand
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  • Open Access
Inhibition of bacterial disulfide bond formation by the anticoagulant warfarin
Blood coagulation in humans requires the activity of vitamin K epoxide reductase (VKOR), the target of the anticoagulant warfarin (Coumadin). Bacterial homologs of VKOR were recently found toExpand
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Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase
Using a component of the Escherichia coli protein degradation machinery, we have established a system to regulate protein stability in mycobacteria. A protein tag derived from the E. coli SsrAExpand
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  • Open Access
Subcellular Localization of the Intracellular Survival-Enhancing Eis Protein of Mycobacterium tuberculosis
ABSTRACT Mycobacterium tuberculosis is a facultative intracellular pathogen that has evolved the ability to survive and multiply within human macrophages. It is not clear how M. tuberculosis avoidsExpand
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The RssAB two-component signal transduction system in Serratia marcescens regulates swarming motility and cell envelope architecture in response to exogenous saturated fatty acids.
Serratia marcescens swarms at 30 degrees C but not at 37 degrees C on a nutrient-rich (LB) agar surface. Mini-Tn5 mutagenesis of S. marcescens CH-1 yielded a mutant (WC100) that swarms not onlyExpand
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Direct and simultaneous identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by rapid multiplex nested PCR-ICT assay.
The Mycobacterium tuberculosis (MTB) shows different virulence and host infection range from other members of the M. tuberculosis complex (MTBC). Differential identification of MTB from MTBC is thusExpand
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