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Distantly related sequences in the alpha‐ and beta‐subunits of ATP synthase, myosin, kinases and other ATP‐requiring enzymes and a common nucleotide binding fold.
TLDR
Related sequences in both alpha and beta and in other enzymes that bind ATP or ADP in catalysis help to identify regions contributing to an adenine nucleotide binding fold in both ATP synthase subunits.
Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels.
TLDR
Investigation of the over-production of seven membrane proteins in an Escherichia coli-bacteriophage T7 RNA polymerase expression system found that when expression of the target membrane protein was induced, most of the BL21(DE3) host cells died.
Molecular architecture of the rotary motor in ATP synthase.
TLDR
An electron density map obtained from crystals of a subcomplex of yeast mitochondrial ATP synthase shows a ring of 10 c subunits whose extensive contact between the c ring and the stalk suggests that they may rotate as an ensemble during catalysis.
The NADH:ubiquinone oxidoreductase (complex I) of respiratory chains.
  • J. Walker
  • Biology, Physics
    Quarterly reviews of biophysics
  • 1 August 1992
The inner membranes of mitochondria contain three multi-subunit enzyme complexes that act successively to transfer electrons from NADH to oxygen, which is reduced to water (Fig. I). The first enzyme
Isolation of a fragment of tau derived from the core of the paired helical filament of Alzheimer disease.
TLDR
A substantially enriched preparation of Alzheimer paired helical filaments has been used as a starting point for biochemical studies and sequence analysis of these peptides was used to design oligonucleotide probes for cloning a cognate cDNA, which leads to its identification as human microtubule-associated tau protein.
Cloning and sequencing of the cDNA encoding a core protein of the paired helical filament of Alzheimer disease: identification as the microtubule-associated protein tau.
TLDR
The proof that at least part of tau protein forms a component of the paired helical filament core opens the way to understanding the mode of formation of paired helicals filaments and thus, ultimately, the pathogenesis of Alzheimer disease.
Modulation of the Oligomerization State of the Bovine F1-ATPase Inhibitor Protein, IF1, by pH*
TLDR
The mutation of histidine 49 to lysine is predicted to abolish coiled-coil formation over residues 32–43 preventing interaction between two dimers, forcing the equilibrium toward the dimeric state, thereby freeing the N-terminal inhibitory regions and allowing them to interact with F1.
Mitochondrial ATP synthase subunit c storage in the ceroid-lipofuscinoses (Batten disease).
TLDR
Findings suggest that ovine ceroid-lipofuscinosis is caused by a specific failure in the degradation of subunit c after its normal inclusion into mitochondria, and its consequent abnormal accumulation in lysosomes.
Citrin and aralar1 are Ca2+‐stimulated aspartate/glutamate transporters in mitochondria
TLDR
The results demonstrate that citrin and aralar1 are isoforms of the hitherto unidentified aspartate/glutamate carrier and explain why mutations in citrin cause type II citrullinemia in humans, and suggest a novel mechanism of Ca2+ regulation of the as partate/malate shuttle.
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