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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Production of single-stranded plasmid DNA.
Use of the red fluorescent protein as a marker of Kaposi's sarcoma-associated herpesvirus lytic gene expression.
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
The Human Cytomegalovirus Chemokine Receptor US28 Mediates Vascular Smooth Muscle Cell Migration
Functional Analysis of the Human Cytomegalovirus US28 Gene by Insertion Mutagenesis with the Green Fluorescent Protein Gene
The ability of the US28 gene to bind chemokines was shown to cause a significant reduction in the concentration of RANTES in the medium of infected cells, demonstrating that US28 is responsible for the β-chemokine binding and induced calcium signaling in HCMV-infected cells.
New pUC-derived cloning vectors with different selectable markers and DNA replication origins.
Human herpesvirus 8 interaction with target cells involves heparan sulfate.
Data show that the gamma2 herpesvirus HHV-8, similar to some members of alpha, beta, and gamma2 genital herpesviruses, adsorbs to cells by binding to cell surface HS-like moieties, and could be the first set of ligand-receptor interaction leading to the binding with one or more host cell receptors essential for the subsequent viral entry process.
Cytomegalovirus Cell Tropism, Replication, and Gene Transfer in Brain
- A. N. van den Pol, E. Mocarski, N. Saederup, J. Vieira, T. J. Meier
- BiologyThe Journal of Neuroscience
- 15 December 1999
Rec recombinant murine cytomegalovirus entered and expressed reporter genes in both rat and human brain cells, suggesting that mCMV might serve as a vector for gene transfer into brain cells of non-murine species.