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Metabolomics technologies and metabolite identification
Metabolomics studies rely on the analysis of the multitude of small molecules (metabolites) present in a biological system. Most commonly, metabolomics is heavily supported by mass spectrometry (MS)Expand
Cladosporium fulvum Avr4 protects fungal cell walls against hydrolysis by plant chitinases accumulating during infection.
In situ fluorescence studies showed that Avr4 also binds to cell walls of C. fulvum during infection of tomato, where it most likely protects the fungus against tomato chitinases, suggesting that AvR4 is a counter-defensive virulence factor. Expand
Regioselectivity of phase II metabolism of luteolin and quercetin by UDP-glucuronosyl transferases.
Regioselectivity is dependent on the model flavonoid of interest, glucuronidation of luteolin and quercetin not following the same pattern, depending on the isoenzyme of UDP-glucuronosyltransferases (UGT), and the human intestine UGT's appear to be especially effective in conjugating this 3',4' catechol unit. Expand
Tissue specialization at the metabolite level is perceived during the development of tomato fruit.
Findings suggest spatio-temporal specificity in the accumulation of endogenous metabolites from tomato fruit, which could be assigned to specific ripening stages or tissues. Expand
The Cladosporium fulvum Virulence Protein Avr2 Inhibits Host Proteases Required for Basal Defense[W][OA]
It is shown that Avr2 is a genuine virulence factor of C. fulvum that inhibits several Cys proteases required for plant basal defense. Expand
A Liquid Chromatography-Mass Spectrometry-Based Metabolome Database for Tomato1
Unbiased metabolic profiling and comparison of peel and flesh tissues from tomato fruits validated the applicability of the MoTo DB, revealing that all flavonoids and α-tomatine were specifically present in the peel, while several other alkaloids and some particular phenylpropanoids were mainly present inThe flesh tissue. Expand
Natural Disulfide Bond-disrupted Mutants of AVR4 of the Tomato Pathogen Cladosporium fulvum Are Sensitive to Proteolysis, Circumvent Cf-4-mediated Resistance, but Retain Their Chitin Binding Ability*
The widely applied tomato Cf-4 resistance gene is circumvented by C. fulvum by amino acid substitutions affecting two disulfide bonds in AVR4 resulting in the absence of the corresponding AVR2 isoforms in apoplastic fluid, but these natural isoforms appear to have retained their intrinsic function. Expand
Automatic chemical structure annotation of an LC-MS(n) based metabolic profile from green tea.
A novel method to automatically process and annotate the LC-MS(n) data sets on the basis of candidate molecules from chemical databases, such as PubChem or the Human Metabolite Database, illustrating the potential to support systematic and untargeted metabolite identification. Expand
Degradation of 3,4‐Dichloro‐ and 3,4‐Difluoroaniline by Pseudomonas fluorescens 26‐K
3,4‐Dichloro‐ and 3, 4‐difluoroanilines were degraded by Pseudomonas fluorescens 26‐K under aerobic conditions and the presence of 3‐chloro‐4‐hydroxyaniline as a metabolite suggested that 3,4-DCA degradation pathway includes dehalogenation and hydroxylation of aromatic ring followed by its subsequent cleaving by C2,3DO. Expand
Identification of 14 quercetin phase II mono- and mixed conjugates and their formation by rat and human phase II in vitro model systems.
It is concluded that none of the in vitro models converted quercetin to a phase II metabolite mixture similar to the in vivo plasma metabolite pattern of quercets, opening the way for a better-funded assessment of the biological activity of quERCetin in various biological systems. Expand