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Reversible methylation of m6Am in the 5′ cap controls mRNA stability
Using a transcriptome-wide map of m6Am, it is found that m 6Am-initiated transcripts are markedly more stable than mRNAs that begin with other nucleotides and that m6 am is selectively demethylated by fat mass and obesity-associated protein (FTO).
La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region
- Lucas Philippe, J. Vasseur, F. Debart, Carson C. Thoreen
- BiologyNucleic acids research
- 13 December 2017
It is shown that the C-terminal half of LARP1 is necessary and sufficient to control TOP mRNA translation in cells and support a model whereby the translation of TOP mRNAs is controlled by a growth-regulated competition between eIF4F and Larp1 for their 5′ ends.
Identiﬁcation of the m 6 Am Methyltransferase PCIF1 Reveals the Location and Functions of m 6 Am in the Transcriptome
The genome was binned into 50 nt non-sliding windows and the coverage of reads in each was counted for each strand, discarding zero-coverage bins and the log 2 ratio of BPM+1 for wild-type to PCIF1 knockout was calculated.
Bacillus subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates
- J. Piton, V. Larue, C. Condon
- Biology, ChemistryProceedings of the National Academy of Sciences
- 22 April 2013
The crystal structures of Bacillus subtilis RppH bound to GTP and to a triphosphorylated dinucleotide RNA are presented, highlighting important structural and functional differences between BsRppH and the RNA deprotection enzymes of distantly related bacteria.
Identification of the m6Am Methyltransferase PCIF1 Reveals the Location and Functions of m6Am in the Transcriptome.
FTO-mediated cytoplasmic m6Am demethylation adjusts stem-like properties in colorectal cancer cell
It is shown that the cytoplasmic pool of fat mass and obesity-associated protein (FTO) impedes CSC abilities in colorectal cancer through its m6Am (N6,2’-O-dimethyladenosine) demethylase activity, bringing to light the first biological function of the m6 am modification and its potential adverse consequences for coloreCTal cancer management.
Molecular Basis for Nucleotide Conservation at the Ends of the Dengue Virus Genome
It is shown that NS5PolDV contains a specific priming site for adenosine 5′-triphosphate as the first transcribed nucleotide in the dengue virus, and that these conservational mechanisms, mediated by the polymerase alone, may extend to other RNA virus families having RdRps initiating RNA synthesis de novo.
X-ray structure and activities of an essential Mononegavirales L-protein domain
A portion of human Metapneumovirus L consisting of CR-VI and the poorly conserved region at its C terminus, the +domain is presented, which sequentially methylates caps at their 2′O and N7 positions, and also displays nucleotide triphosphatase activity.
Cap-proximal nucleotides via differential eIF4E binding and alternative promoter usage mediate translational response to energy stress
Several levels of coordination of transcription and translation responses to energy stress are uncovered, including novel glucose starvation-induced downstream transcripts for the translation regulators eIF4A and Pabp, which are also translationally-induced despite general translational inhibition.
mRNA Capping by Venezuelan Equine Encephalitis Virus nsP1: Functional Characterization and Implications for Antiviral Research
The capping of a 5′ diphosphate synthetic RNA mimicking the 5′ end of an alphavirus mRNA was observed in vitro for the first time and the inhibitory effects of sinefungin, aurintricarboxylic acid, and ribavirin triphosphate on MTase and capping reactions were investigated, providing possible avenues for antiviral research.