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Condition-Dependent Transcriptome Reveals High-Level Regulatory Architecture in Bacillus subtilis
Outside In Acquisition and analysis of large data sets promises to move us toward a greater understanding of the mechanisms by which biological systems are dynamically regulated to respond toExpand
Signal Peptide-Dependent Protein Transport inBacillus subtilis: a Genome-Based Survey of the Secretome
SUMMARY One of the most salient features of Bacillus subtilis and related bacilli is their natural capacity to secrete a variety of proteins into their environment, frequently to high concentrations.Expand
Essential Bacillus subtilis genes
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the organism,Expand
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
SUMMARY Secretory proteins perform a variety of important“ remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to makeExpand
A proteomic view on genome-based signal peptide predictions.
The availability of complete genome sequences has allowed the prediction of all exported proteins of the corresponding organisms with dedicated algorithms. Even though numerous studies report onExpand
Identification of VceA and VceC, two members of the VjbR regulon that are translocated into macrophages by the Brucella type IV secretion system
Survival and replication inside host cells by Brucella spp. requires a type IV secretion system (T4SS), encoded by the virB locus. However, the identity of the molecules secreted by the T4SS hasExpand
Type I signal peptidases of Gram-positive bacteria.
Proteins that are exported from the cytoplasm to the periplasm and outer membrane of Gram-negative bacteria, or the cell wall and growth medium of Gram-positive bacteria, are generally synthesized asExpand
Two minimal Tat translocases in Bacillus
Activity of the Tat machinery for protein transport across the inner membrane of Escherichia coli and the chloroplast thylakoidal membrane requires the presence of three membrane proteins: TatA, TatBExpand
Genome engineering reveals large dispensable regions in Bacillus subtilis.
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,Expand
Mapping the Pathways to StaphylococcalPathogenesis by Comparative Secretomics
SUMMARY The gram-positive bacterium Staphylococcus aureus is a frequent component of the human microbial flora that can turn into a dangerous pathogen. As such, this organism is capable of infectingExpand