HIV-1 Integrase Forms Stable Tetramers and Associates with LEDGF/p75 Protein in Human Cells*
The findings indicate that the minimal IN molecule in human cells is a homotetramer, suggesting that at least an octamer of IN is required to accomplish coordinated integration of both retroviral long terminal repeats and that LEDGF is a cellular factor involved in this process.
Type II Metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana Cleave Substrates after Arginine and Lysine*
- D. Vercammen, B. van de Cotte, F. Van Breusegem
- BiologyJournal of Biological Chemistry
- 29 October 2004
Detailed biochemical characterization with a broad range of synthetic oligopeptides and several protease inhibitors of purified recombinant proteins of both metacaspases showed that both metACaspases are arginine/lysine-specific cysteine proteases and did not cleave caspase-specific synthetic substrates.
Molecular Phenotyping of the pal1 and pal2 Mutants of Arabidopsis thaliana Reveals Far-Reaching Consequences on Phenylpropanoid, Amino Acid, and Carbohydrate Metabolism
Together, from the molecular phenotype, common and specific functions of PAL1 and PAL2 are delineated, and PAL1 is qualified as being more important for the generation of phenylpropanoids.
Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus.
- N. Parry, D. Beever, E. Owen, I. Vandenberghe, J. V. Van Beeumen, M. Bhat
- Biology, ChemistryBiochemical Journal
The stereochemistry of cellobiose hydrolysis revealed that beta-glucosidase from T. aurantiacus is a retaining glycosidase, while N-terminal amino acid sequence alignment indicated that it is a member of glycoside hydrolase family 3.
Determination of the gene sequence and the molecular structure of the enterococcal peptide antibiotic AS-48
It is proposed that the AS-48 translated primary product is very likely submitted to a posttranslational modification during secretion by an atypical or a typical signal peptidase that cleaves off a 35-residue or shorter signal peptide from the prepeptide molecule and by the linkage of the methionine residue to the C-terminal tryptophan residue to obtain the cyclic peptide.
The microneme protein MIC3 of Toxoplasma gondii is a secretory adhesin that binds to both the surface of the host cells and the surface of the parasite
Results suggest that MIC3 is a new microneme adhesin of T. gondii, which can be involved in protein–protein or protein–carbohydrate interactions.
The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan‐polymerizing penicillin‐binding protein 1b of Escherichia coli
- M. Terrak, Tushar K. Ghosh, M. Nguyen-Distèche
- Biology, ChemistryMolecular Microbiology
- 1 October 1999
In in vitro assays with the lipid precursor and in the presence of penicillin at concentrations sufficient to derivatize the active‐site serine 510 of the acyl transferase, the rate of glycan chain synthesis is unmodified, showing that the functioning of the glycosyl transfer enzyme is acyltransferase independent.
Cell-Wall-Bound Proteinase of Lactobacillus delbrueckii subsp. lactis ACA-DC 178: Characterization and Specificity for β-Casein
- E. Tsakalidou, R. Anastasiou, I. Vandenberghe, J. V. Van Beeumen, G. Kalantzopoulos
- Medicine, BiologyApplied and Environmental Microbiology
- 1 May 1999
ABSTRACT Lactobacillus delbrueckii subsp. lactisACA-DC 178, which was isolated from Greek Kasseri cheese, produces a cell-wall-bound proteinase. The proteinase was removed from the cell envelope by…
The protein composition of honeybee venom reconsidered by a proteomic approach.
Isolation and biochemical characterisation of enterocins produced by enterococci from different sources.
- M. R. Foulquié Moreno, R. Callewaert, B. Devreese, J. V. Van Beeumen, L. De Vuyst
- Medicine, BiologyJournal of Applied Microbiology
- 1 February 2003
AIMS Comparison of enterocins produced by six Enterococcus faecium strains and one Ent. faecalis strain isolated from different origin with regard to their microbiological and biochemical…