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Temperature dependent expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis
Results showed that the abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary sperMatocytes, but it blocked the translation of them, and it was found that testosterone could regulate the Cyclin B 1 expression in sper matogenic cells.
Expression of regulator of G-protein signalling protein 2 (RGS2) in the mouse uterus at implantation sites.
Results indicate that RGS2 has a role during implantation, possibly by regulating the intracellular Ca(2+) mobilization and T-cell proliferation at the maternal-fetal interface.
Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage
The results suggest that oviductin has a function not only on fertilization, but also on the release of early embryonic development block, and the later function domain of rabbit oVIDuctin may be situate in its C-terminal.
In vivo effects of microinjected alkaline phosphatase and its low molecular weight substrates on the first meiotic cell division in Xenopus laevis oocytes.
The results indicate that phosphate or phosphoester-containing buffers should be avoided in the course of maturation-promoting factor purification, and alkaline phosphatase might behave in vivo as a phosphoprotein phosphat enzyme active towards phosphotyrosine-containing proteins.
Microinjected progesterone reinitiates meiotic maturation of Xenopus laevis oocytes.
Progesterone acts initially on an intracellular site in order to trigger meiotic maturation of the Xenopus oocyte, and microinjected progesterone decreases the cAMP content as does externally applied hormone.
Monensin blocks the first meiotic cell division of the Xenopus oocyte: Effect at the nuclear membrane level
Observations show that monensin, which is known to abolish intracellular proton gradients, interferes with the mechanism of the breakdown of the nuclear envelope induced by MPF.
A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation
ABSTRACTp28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13suc1-agarose affinity chromatography. Sequence homology analysis of the full-length cDNA of p28(Gene
Low temperature induces oocytes p34cdc2 synthesis and accumulation- the acquisition of competence to resume meiosis in toad oocytes1
Results indicated that a low temperature environment is essential for the oocytes of Bufo Bufe gargarizans to express and store some cell cycle drivers and its regulators, and to gain the maturation competence.