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Genomic Imprinting Disrupted by a Maternal Effect Mutation in the Dnmt1 Gene
Transient nuclear localization of Dnmt1o in 8-cell embryos suggests that this variant of DNmt1 provides maintenance methyltransferase activity specifically at imprinted loci during the fourth embryonic S phase. Expand
Methylation dynamics of imprinted genes in mouse germ cells.
It is demonstrated that maternal methylation imprints on at least one imprinted gene, Snrpn, are established during the postnatal growth phase of oogenesis, which raises the possibility that assisted reproductive technologies that involve in vitro maturation of oocytes may result in developmental abnormalities due to incomplete methylated imprints in immature oocytes. Expand
Gene-specific timing and epigenetic memory in oocyte imprinting.
The methylation of the Snrpn, Igf2r, Peg1 and Peg3 differentially methylated regions in postnatal growing mouse oocytes indicated that methylation was acquired asynchronously at these different genes, contributing to the understanding of the epigenetic mechanisms underlying imprint acquisition during female germ cell development and for the practice of assisted reproductive technologies. Expand
Superovulation alters the expression of imprinted genes in the midgestation mouse placenta.
The results show that both maternally and paternally methylated imprinted imprinted genes were affected, suggesting that superovulation compromises oocyte quality and interferes with the maintenance of imprinting during preimplantation development. Expand
Sex-specific exons control DNA methyltransferase in mammalian germ cells.
The spermatozoon and oocyte genomes bear sex-specific methylation patterns that are established during gametogenesis and are required for the allele-specific expression of imprinted genes in somaticExpand
Transcription factor GATA-4 is expressed in a sexually dimorphic pattern during mouse gonadal development and is a potent activator of the Müllerian inhibiting substance promoter.
Analysis of the ontogeny of gonadal GATA-4 expression by immunohistochemistry found that the Müllerian inhibiting substance promoter which harbors a conserved GATA element is a downstream target for Gata-4, indicating a new factor in the cascade of regulators that control gonadal development and sex differentiation in mammals. Expand
Dynamics of Dnmt1 methyltransferase expression and intracellular localization during oogenesis and preimplantation development.
The highly restricted nuclear localization patterns of oocyte-derived Dnmt1o and DnMT1 during preimplantation development add further support to the notion that DNA methyltransferases other than Dn mt1 are required for maintaining imprints during pre- and postimplantations development. Expand
Acquisition of the H19 methylation imprint occurs differentially on the parental alleles during spermatogenesis.
A temporal analysis of the methylation patterns at the H19 locus during postnatal male germ cell development suggests that methylation is acquired in this region prior to the resumption of mitosis in postnatalmale mice. Expand
Rare congenital disorders, imprinted genes, and assisted reproductive technology
Three recent studies all report an unexpectedly high incidence of Beckwith-Wiedemann syndrome (BWS) in children conceived with ARTs. Expand
Windows for sex-specific methylation marked by DNA methyltransferase expression profiles in mouse germ cells.
The results suggest that the mechanism of meiotic down-regulation differs between the sexes and provide clues to specific roles for the different DNMT family members in de novo and maintenance methylation in the developing testis and ovary. Expand