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Different Hormonal Regulation of Cellular Differentiation and Function in Nucellar Projection and Endosperm Transfer Cells: A Microdissection-Based Transcriptome Study of Young Barley Grains1[W]
It is shown that probes generated from fixed and embedded tissue sections represent largely the transcriptome of nonchemically treated and nonamplified references, and up-regulation of amino acid permeases in ETC indicates important roles in active nutrient uptake from the apoplastic space into the endosperm. Expand
Development of endosperm transfer cells in barley
  • J. Thiel
  • Biology, Medicine
  • Front. Plant Sci.
  • 26 March 2014
A comprehensive morphological analysis depicted distinct developmental time points in establishment of transfer cell (TC) morphology and revealed intracellular changes possibly associated with cell wall metabolism and an essential role for two-component signaling (TCS) pathways in ETC development of barley emerged. Expand
Amino acid metabolism at the maternal–filial boundary of young barley seeds: a microdissection-based study
The established micromethods revealed strategies in NP of young barley grains for mobilization and metabolism of transient N and S reserves and transfer into the endosperm. Expand
Seed-specific elevation of non-symbiotic hemoglobin AtHb1: beneficial effects and underlying molecular networks in Arabidopsis thaliana
Overexpression of AtHb1 shifted seed metabolism to an energy-saving mode with the most prominent alterations occurring in cell wall metabolism, and improves oxidative stress tolerance compared to the wild type where a strong transcriptional and metabolic reconfiguration was observed in the hypoxic response. Expand
Caspase-Like Activities Accompany Programmed Cell Death Events in Developing Barley Grains
The distribution of the TUNEL-positive nuclei, tissues-specific expression of genes encoding proteases with potential caspase activities and cascades of casp enzyme-like activities suggest that each seed tissue follows individual pattern of development and disintegration, which however harmonizes with growth of the other tissues in order to achieve proper caryopsis development. Expand
Differentiation of endosperm transfer cells of barley: a comprehensive analysis at the micro-scale.
Gene expression analysis indicated that ethylene-signalling pathways initiate ETC morphology, and transcriptional activity of assimilate and micronutrient transporters suggests ETCs as the main uptake organs of solutes into the endosperm, which grows maximally after E TCs are fully developed. Expand
454 Transcriptome Sequencing Suggests a Role for Two-Component Signalling in Cellularization and Differentiation of Barley Endosperm Transfer Cells
The findings suggest an integral function for the TCS in ETC differentiation possibly coupled to sequent hormonal regulation by ABA and ethylene, and TCS signalling. Expand
Spatio-Temporal Dynamics of Fructan Metabolism in Developing Barley Grains[W]
The tight partitioning of oligofructan biosynthesis hints at distinct functions of the various fructan types in the young endosperm prior to starch accumulation and in the endOSperm transfer cells that accomplish the assimilate supply toward theendosperm at the storage phase. Expand
Laser-capture microdissection of developing barley seeds and cDNA array analysis of selected tissues.
Methods allowing tissue-specific gene expression profiling of developing barley seeds are reported about, with particular emphasis on the fidelity of transcript data obtained by the developed methods in relation to the in vivo transcriptome. Expand
Gibberellin-to-abscisic acid balances govern development and differentiation of the nucellar projection of barley grains
Summary Hormonal balances of abscisic acid-to-gibberellic acid govern the development and differentiation of the nucellar projection, the maternal organ of barley grains involved in assimilateExpand