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Mechanisms of cell membrane electropermeabilization: a minireview of our present (lack of ?) knowledge.
Cell electropulsation is routinely used in cell Biology for protein, RNA or DNA transfer. Its clinical applications are under development for targeted drug delivery and gene therapy. Nevertheless,… Expand
Electropermeabilization of mammalian cells to macromolecules: control by pulse duration.
Membrane electropermeabilization to small molecules depends on several physical parameters (pulse intensity, number, and duration). In agreement with a previous study quantifying this phenomenon in… Expand
Direct visualization at the single-cell level of electrically mediated gene delivery
- M. Golzio, J. Teissié, M. Rols
- Medicine, Biology
- Proceedings of the National Academy of Sciences…
- 29 January 2002
Electropermeabilization is one of the nonviral methods successfully used to transfer genes into living cells in vitro and in vivo. Although this approach shows promise in the field of gene therapy,… Expand
An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization.
When applied on intact cell suspension, electric field pulses are known to induce membrane permeabilization (electropermeabilization) and fusion (electrofusion). These effects are triggered through a… Expand
FTY720 (fingolimod) sensitizes prostate cancer cells to radiotherapy by inhibition of sphingosine kinase-1.
Radiotherapy is widely used as a radical treatment for prostate cancer, but curative treatments are elusive for poorly differentiated tumors where survival is just 15% at 15 years. Dose escalation… Expand
Sphingosine kinase-1 as a chemotherapy sensor in prostate adenocarcinoma cell and mouse models.
Systemic chemotherapy was considered of modest efficacy in prostate cancer until the recent introduction of taxanes. We took advantage of the known differential effect of camptothecin and docetaxel… Expand
Control by pulse parameters of electric field-mediated gene transfer in mammalian cells.
- H. Wolf, M. P. Rols, E. Boldt, E. Neumann, J. Teissié
- Biology, Medicine
- Biophysical journal
- 1 February 1994
Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). The effect of these parameters… Expand
Electropermeabilization of mammalian cells. Quantitative analysis of the phenomenon.
Transient membrane permeabilization by application of high electric field intensity pulses on cells (electropermeabilization) depends on several physical parameters associated with the technique… Expand
Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane.
Interaction of two stains (propidium iodide and ethidium bromide) with electropermeabilized living Chinese hamster ovary cells is observed using an ultrafast fluorescence image acquisition system.… Expand
What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues
- J. Escoffre, Thomas Portet, L. Wasungu, J. Teissié, D. Dean, M. Rols
- Biology, Medicine
- Molecular biotechnology
- 1 March 2009
Cell membranes can be transiently permeabilized under application of electric pulses. This treatment allows hydrophilic therapeutic molecules, such as anticancer drugs and DNA, to enter into cells… Expand