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Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles
The ability to generate preparative amounts of HPV16 L1 and L1-L2 VLP may have implications for the development of a serological assay to detect anti-HPV16 virion immune responses to conformational epitopes and for immunoprophylaxis against HPV16 infection.
Dose dependency of aflatoxin B1 binding on human high molecular weight DNA in the activation of proto-oncogene.
Excessive AFB1 binding on the hHC and PLC HMW DNAs resulted in an "over-kill" of both cell transformation capability and templating activity of the DNA.
A human hepatocellular carcinoma 3.0-kilobase DNA sequence transforms both rat liver cells and NIH3T3 fibroblasts and encodes a 52-kilodalton protein.
The high-level production of a slightly modified form of this 52-kilodalton protein in a bacterial expression system has been successfully achieved and was similar in electrophoretic behavior to the 52- to 53-kilobase protein synthesized in a cell-free translation system using rabbit reticulocyte lysate programmed with hybrid-selected hhcM-specific mRNA from Mahlavu hepatocellular carcinoma cells.
Transforming DNA sequences of human hepatocellular carcinomas, their distribution and relationship with hepatitis B virus sequence in human hepatomas.
The results suggest that HBV contributes to hepatocarcinogenesis probably via an activation mechanism involving possibly an integration or transient interaction of HBV DNA with hepatocyte DNA sequences, leading to recombination and eventual amplifications of the hhcM sequence in Mahlavu.
Biological activity of cloned rat endogenous C-type virus DNA transferred by microinjection.
The biological activity of a molecularly cloned DNA of a rat endogenous C-type leukaemia helper virus, RHHV, was assessed by intranuclear microinjection into normal rat kidney cells (NRK153) and genome rescue experiments indicated that both the total and the 5.8 to 6.2 kb DNA fragment proximal to the 5' terminus of the cloned RHHVs were able to rescue successfully a transforming replication-competent pseudotype virus.
Activation and Specificity of Aflatoxin B 1 Binding on hhc M , a Human Oncogene of Low Efficiency, and Cell Transformation
Aflatoxin B1 (AFB1), a metabolite of Aspergillus flavus, chemically classified as a furocoumarin, is known to be the most potent hepatocarcinogen experimentally tested in various species including