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The RNA polymerase II core promoter.
TLDR
Although core promoter structure was originally thought to be invariant, a remarkable degree of diversity has become apparent and this article reviews the structural and functional diversity of the RNA polymerase II core promoter. Expand
Tools for neuroanatomy and neurogenetics in Drosophila
TLDR
The results suggest that the D. melanogaster genome contains >50,000 enhancers and that multiple enhancers drive distinct subsets of expression of a gene in each tissue and developmental stage. Expand
The RCAF complex mediates chromatin assembly during DNA replication and repair
TLDR
Genetic analyses in Saccharomyces cerevisiae demonstrate that ASF1 is essential for normal cell cycle progression, and suggest that RCAF mediates chromatin assembly after DNA replication and the repair of double-strand DNA damage in vivo. Expand
Intrinsic histone-DNA interactions are not the major determinant of nucleosome positions in vivo
TLDR
The results argue against a genomic code for nucleosome positioning, and they suggest that the nucleosomal pattern in coding regions arises primarily from statistical positioning from a barrier near the promoter that involves some aspect of transcriptional initiation by RNA polymerase II. Expand
The RNA polymerase II core promoter: a key component in the regulation of gene expression.
TLDR
The development, growth, and survival of eukaryotic organisms require the proper regulation of tens of thousands of genes, and one of these regulatory components is the RNA polymerase II core promoter. Expand
The downstream core promoter element, DPE, is conserved from Drosophila to humans and is recognized by TAFII60 of Drosophila.
TLDR
These studies provide evidence for a direct role of TAFs in basal transcription of TATA-less DPE-containing genes and collectively indicate that the DPE is, in many respects, a downstream counterpart to the TATA box that is present in Drosophila to humans. Expand
ACF, an ISWI-Containing and ATP-Utilizing Chromatin Assembly and Remodeling Factor
TLDR
It is suggested that ACF acts catalytically both in chromatin assembly and in the remodeling of nucleosomes that occurs during transcriptional activation. Expand
Affinity purification of sequence-specific DNA binding proteins.
TLDR
This method provides a means for the purification of rare sequence-specific DNA binding proteins, such as Sp1 and CAAT-binding transcription factor, by using tandem affinity columns containing different protein binding sites. Expand
Regulation of gene expression via the core promoter and the basal transcriptional machinery.
TLDR
The findings suggest that the core promoter and basal transcription factors are important yet mostly unexplored components in the regulation of gene expression. Expand
The Downstream Promoter Element DPE Appears To Be as Widely Used as the TATA Box in Drosophila Core Promoters
TLDR
Data reveal that the DPE exhibits a strict spacing requirement yet some sequence flexibility and appears to be as widely used as the TATA box in Drosophila. Expand
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