In vitro selection of RNA molecules that bind specific ligands
Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules. Roughly one in 1010 random sequence RNA…
The double-strand-break repair model for recombination
A mutant with a defect in telomere elongation leads to senescence in yeast
Yeast transformation: a model system for the study of recombination.
- T. Orr-Weaver, J. Szostak, R. Rothstein
- BiologyProceedings of the National Academy of Sciences…
- 1 October 1981
Consideration of models for plasmid integration and gene conversion suggests that RAD52 may be involved in the DNA repair synthesis required for these processes and implications for the isolation of integrative transformants, fine-structure mapping, and the cloning of mutations are discussed.
A DNA aptamer that binds adenosine and ATP.
A model of the ATP-binding DNA structure which is based on a stable framework composed of two stacked G- Quartets is proposed, which may stack between the top G-quartet and the two short stems, forming a pocket in which the adenosine or ATP ligand binds.
Isolation of new ribozymes from a large pool of random sequences [see comment].
An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules, leading to improvement of the average ligation activity and the emergence of ribozymes with reaction rates 7 million times faster than the uncatalyzed reaction rate.
DNA sequences of telomeres maintained in yeast
Yeast chromosomal telomeres terminate in a DNA sequence consisting of tandem irregular repeats of the general form C1–3A, in an apparently non-template-directed manner, during their replication on linear plasmids in yeast.
In vitro selection of functional nucleic acids.
By selecting high-affinity and -specificity nucleic acid ligands for proteins, promising new therapeutic and diagnostic reagents have been identified and the existence of such RNA enzymes supports the notion that ribozymes could have directed a primitive metabolism before the evolution of protein synthesis.
Double-strand breaks at an initiation site for meiotic gene conversion
It is shown that a double-strand break appears at the ARG4 recombination initiation site at the time of recombination, and that the broken DNA molecules end in long single-stranded tails.
One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.
- A. Keefe, D. Wilson, B. Seelig, J. Szostak
- Biology, ChemistryProtein Expression and Purification
- 1 December 2001
It is demonstrated that a single-step purification of SBP-tagged proteins from bacterial extract yields samples that are more pure than those purified using maltose-binding protein or the His-tag.