Author pages are created from data sourced from our academic publisher partnerships and public sources.
In vitro selection of RNA molecules that bind specific ligands
Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules. Roughly one in 1010 random sequence RNA… Expand
The double-strand-break repair model for recombination
Gene conversion is the nonreciprocal transfer of information from one DNA duplex to another; in meiosis, it is frequently associated with crossing-over. We review the genetic properties of meiotic… Expand
A mutant with a defect in telomere elongation leads to senescence in yeast
We describe a general assay designed to detect mutants of yeast that are defective for any of several aspects of telomere function. Using this assay, we have isolated a mutant that displays a… Expand
A DNA aptamer that binds adenosine and ATP.
We have used in vitro selection to isolate adenosine/ATP-binding DNA sequences from a pool of approximately 2 x 10(14) different random-sequence single-stranded DNA molecules. One of these aptamers… Expand
Isolation of new ribozymes from a large pool of random sequences [see comment].
An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules. These ribozymes ligate two RNA molecules… Expand
Yeast transformation: a model system for the study of recombination.
- T. Orr-Weaver, J. Szostak, R. J. Rothstein
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 1 October 1981
DNA molecules that integrate into yeast chromosomes during yeast transformation do so by homologous recombination. We have studied the way in which circular and linear molecules recombine with… Expand
One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.
- A. Keefe, D. Wilson, B. Seelig, J. Szostak
- Chemistry, Medicine
- Protein expression and purification
- 1 December 2001
We describe the use of the SBP-tag, a new streptavidin-binding peptide, for both the one-step purification and the detection of recombinant proteins. The SBP-tag sequence is 38 amino acids long and… Expand
In vitro selection of functional nucleic acids.
In vitro selection allows rare functional RNA or DNA molecules to be isolated from pools of over 10(15) different sequences. This approach has been used to identify RNA and DNA ligands for numerous… Expand
HIV-1 rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA
We have used an iterative in vitro genetic selection to identify the important structural features of the viral RNA element bound by the Rev protein of human immunodeficiency virus type 1 (HIV-1).… Expand
DNA sequences of telomeres maintained in yeast
Telomeres, the ends of eukaryotic chromosomes, have long been recognized as specialized structures. Their stability compared with broken ends of chromosomes1,2 suggested that they have properties… Expand