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In vitro selection of RNA molecules that bind specific ligands
Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules. Roughly one in 1010 random sequence RNA…
The double-strand-break repair model for recombination
This work proposes a new mechanism for meiotic recombination, in which events are initiated by double-strand breaks that are enlarged to double- Strand gaps, and postmeiotic segregation can result from heteroduplex DNA formed at the boundaries of the gap-repair region.
A mutant with a defect in telomere elongation leads to senescence in yeast
Using this assay, a mutant that displays a progressive decrease in telomere length as well as an increased frequency of chromosome loss is isolated, which defines a new gene, designated EST1 (for ever shorter telomeres).
Yeast transformation: a model system for the study of recombination.
- T. Orr-Weaver, J. Szostak, R. Rothstein
- Biology, MedicineProceedings of the National Academy of Sciences…
- 1 October 1981
Consideration of models for plasmid integration and gene conversion suggests that RAD52 may be involved in the DNA repair synthesis required for these processes and implications for the isolation of integrative transformants, fine-structure mapping, and the cloning of mutations are discussed.
Isolation of new ribozymes from a large pool of random sequences [see comment].
An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules, leading to improvement of the average ligation activity and the emergence of ribozymes with reaction rates 7 million times faster than the uncatalyzed reaction rate.
A DNA aptamer that binds adenosine and ATP.
A model of the ATP-binding DNA structure which is based on a stable framework composed of two stacked G- Quartets is proposed, which may stack between the top G-quartet and the two short stems, forming a pocket in which the adenosine or ATP ligand binds.
DNA sequences of telomeres maintained in yeast
Yeast chromosomal telomeres terminate in a DNA sequence consisting of tandem irregular repeats of the general form C1–3A, in an apparently non-template-directed manner, during their replication on linear plasmids in yeast.
In vitro selection of functional nucleic acids.
By selecting high-affinity and -specificity nucleic acid ligands for proteins, promising new therapeutic and diagnostic reagents have been identified and the existence of such RNA enzymes supports the notion that ribozymes could have directed a primitive metabolism before the evolution of protein synthesis.
One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.
- A. Keefe, D. Wilson, B. Seelig, J. Szostak
- Chemistry, MedicineProtein expression and purification
- 1 December 2001
It is demonstrated that a single-step purification of SBP-tagged proteins from bacterial extract yields samples that are more pure than those purified using maltose-binding protein or the His-tag.
HIV-1 rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA
It is proposed that the G:G base pair distorts the sugar-phosphate backbone of viral RNA and that this distortion is a critical determinant of recognition by Rev.