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Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination…
The Role of Spatially Controlled Cell Proliferation in Limb Bud Morphogenesis
Oriented cell behaviors likely have a more important role in limb bud elongation during development than previously suggested by the “growth-based morphogenesis” hypothesis.
Multi-view image fusion improves resolution in three-dimensional microscopy.
A non-blind, shift-invariant image processing technique that fuses multi-view three-dimensional image data sets into a single, high quality three-dimensional image is presented. It is effective for…
High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy
- P. Verveer, J. Swoger, F. Pampaloni, K. Greger, Marco Marcello, E. Stelzer
- Biology, PhysicsNature Methods
- 1 April 2007
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal…
Basic building units and properties of a fluorescence single plane illumination microscope.
The basic principles underlying EMBL's SPIM and its unique properties such as the efficient usage of the fluorophores, the reduced photo toxic effects, the true optical sectioning capability, and the excellent axial resolution are described.
Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
This study demonstrates that a defined chromosomal structure is a universal feature of living systems and provides evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization.
pMHC affinity controls duration of CD8+ T cell–DC interactions and imprints timing of effector differentiation versus expansion
This work uses intravital two-photon microscopy and quantitative whole-organ imaging to reveal the dynamics of early affinity-driven CD8+ T cell activation.
Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions.
It is observed that despite a 20-fold reduction of chemokine expression in virus-challenged PLNs, CXCR5 remained essential for B-cell screening of antigen-presenting cells and shed light on the local chemokines availability that these cells compute.
Decrease in Cell Volume Generates Contractile Forces Driving Dorsal Closure.
OPTiSPIM: integrating optical projection tomography in light sheet microscopy extends specimen characterization to nonfluorescent contrasts.
- J. Mayer, A. Robert-Moreno, Renzo Danuser, J. Stein, J. Sharpe, J. Swoger
- BiologyOptics letters
- 15 February 2014
A hybrid setup (OPTiSPIM) combining the advantages of each technique and the combination of fluorescent and nonfluorescent high-resolution 3D data into integrated datasets enables a more extensive representation of mesoscopic biological samples.