Shaping cups into phagosomes and macropinosomes
- J. Swanson
- BiologyNature reviews. Molecular cell biology
- 1 August 2008
Recent studies, focusing on the physical properties of forming cups, indicate that a feedback mechanism regulates the signal transduction of phagocytosis and macropinocyTosis.
A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages
It is shown that PI 3-kinase is not necessary for receptor-mediated stimulation of pseudopod extension, but rather functions in the closure of macropinosomes and phagosomes into intracellular organelles.
pH-dependent regulation of lysosomal calcium in macrophages.
P pH-corrected measurements of free calcium concentrations in lysosomes of mouse macrophages are reported, indicating thatLysosomal calcium concentration is high and is maintained in part by the proton gradient across lysOSomal membranes.
Fluorescence resonance energy transfer-based stoichiometry in living cells.
Cdc42, Rac1, and Rac2 display distinct patterns of activation during phagocytosis.
These distinct roles for Cdc42, Rac1, and Rac2 in the component activities of phagocytosis indicate mechanisms by which their differential regulation coordinates rearrangements of actin and membranes.
Salmonella typhimurium activates virulence gene transcription within acidified macrophage phagosomes.
- C. A. Alpuche Aranda, J. Swanson, W. P. Loomis, S. Miller
- BiologyProceedings of the National Academy of Sciences…
- 1 November 1992
Observations implicate phagosome acidification as an intracellular inducer of PhoP-regulated gene expression and suggest that Salmonella survival is dependent on its ability to attenuate phagosomes acidification.
Detection of prokaryotic mRNA signifies microbial viability and promotes immunity
This corrects the article to show that the method used to derive the H2O2 “spatially aggregating force” is based on a two-step process, not a single step, like in the previous version of this paper.
Because macropinosomes are relatively large, they provide an efficient route for non-selective endocytosis of solute macromolecules, this route may facilitate MHC-class-II-restricted antigen presentation by dendritic cells.
The Listeria monocytogenes hemolysin has an acidic pH optimum to compartmentalize activity and prevent damage to infected host cells
- I. Glomski, M. Gedde, A. W. Tsang, J. Swanson, D. Portnoy
- BiologyJournal of Cell Biology
- 18 March 2002
The data show that the acidic pH optimum of LLO results from an adaptive mutation that acts to limit cytolytic activity to acidic vesicles and prevent damage in the host cytosol, a strategy also used by host cells to compartmentalize lysosomal hydrolases.